{"refrec":{"BRefID":59412,"RR":"<b>Granbom, M.; Chow, F.; Lopes, P.F.; de Oliveira, M.C.; Colepicolo, P.; de Paula, E.J.; Pedersén, M.</b> (2004). Characterisation of nitrate reductase in the marine macroalga <i>Kappaphycus alvarezii</i> (Rhodophyta). <i>Aquat. Bot. 78(4)</i>: 295-305. <a href=\"http://dx.doi.org/10.1016/j.aquabot.2003.11.001\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquabot.2003.11.001</a>","BEntID":57002,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Bot. 78(4)</i>: 295-305. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2003.11.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2003.11.001</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Granbom, M.; Chow, F.; Lopes, P.F.; de Oliveira, M.C.; Colepicolo, P.; de Paula, E.J.; Pedersén, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Granbom, M. <i>et al.</i>","Englishabstract":"Nitrate reductase (NR) of the marine red macroalga <i>Kappaphycus alvarezii</i> (Doty) Doty ex P. Silva was examined regarding the biochemical characteristics and daily fluctuations in activity. NR activity was maximum at pH 8.0 and stable over a wide range of temperatures, with an optimum at 23 °C. The highest specific activity measured in a crude extract was 0.16 U mg<sup>-1</sup> protein. Apical parts of the algal thallus showed 1.5 times higher activity compared to basal parts. The NR was NADH-specific (EC 1.6.6.1), since no activity was found when NADPH was used as an electron donor. The apparent Michaelis-Menten (K<sub>M</sub>) constants for nitrate and NADH were 30 and 5 µM, respectively. The stability of NR was assessed by storing the crude extract in different temperatures. Stored at 4 °C, the crude extract maintained high NR activity for 1 week. NR activity exhibited a daily rhythm in algae grown under either a light-dark cycle of 55 µmol photons m<sup>-2</sup> s<sup>-1</sup> or in constant light of 100 µmol photons m<sup>-2</sup> s<sup>-1</sup>, with maximal activity during the midday phase. 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