Document of bibliographic reference 59763

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

The testosterone metabolism of the estuarine invertebrate Neomysis integer (Crustacea: Mysidacea): Identification of testosterone metabolites and endogenous vertebrate-type steroids

Abstract

Testosterone metabolism by Neomysis integer (Crustacea; Mysidacea) was assessed to obtain initial data on its metabolic capacity. N. integer were exposed to both testosterone and [₁₄]testosterone. Identification of testosterone metabolites and endogenous steroids was performed using thin-layer chromatography and liquid chromatography with multiple mass spectrometry. Endogenous production of testosterone in mysids was detected for the first time. N. integer were exposed to testosterone and metabolized administered testosterone extensively. At least 11 polar testosterone metabolites (R^f,metabolite < R^{f,testosterone}), androstenedione, dihydrotestosterone, and testosterone were produced in vivo by N. integer. A sex-specific testosterone metabolism was also observed, although this observation requires further confirmation. The anabolic steroid ß-boldenone was also identified for the first time in invertebrates. The metabolic pathway leading to the formation of ß-boldenone remains unknown, since the steroidal precursor androstadienedione could not be detected. These results reveal interesting similarities in enzyme systems in invertebrate and vertebrate species. Alterations in steroid hormone metabolism may be used as a new biomarker for the effects of endocrine disruptors in invertebrates.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000175611900009

Bibliographic citation

Verslycke, T.; De Wasch, K.; De Brabander, H.F.; Janssen, C.R. (2002). The testosterone metabolism of the estuarine invertebrate Neomysis integer (Crustacea: Mysidacea): Identification of testosterone metabolites and endogenous vertebrate-type steroids. Gen. Comp. Endocrinol. 126: 190-199. http://dx.doi.org/10.1006/gcen.2002.7793

Topic

Brakish water

Is peer reviewed

true