{"refrec":{"BRefID":62189,"RR":"<b>Hoarau, P.; Garello, G.; Gnassia-Barelli, M.; Roméo, M.; Girard, J.-P.</b> (2004). Effect of three xenobiotic compounds on glutathione S-transferase in the clam <i>Ruditapes decussatus</i>. <i>Aquat. Toxicol. 68(1)</i>: 87-94. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2004.03.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2004.03.001</a>","BEntID":58818,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 68(1)</i>: 87-94. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2004.03.001\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2004.03.001</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Hoarau, P.; Garello, G.; Gnassia-Barelli, M.; Roméo, M.; Girard, J.-P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Hoarau, P. <i>et al.</i>","Englishabstract":"The effects of 4,4′DDE, methoxychlor and imidazole were studied on glutathione S-transferase activities in the gills and hepatopancreas of the clam <i>Ruditapes decussatus</i>. The contamination doses were 0.14 µM for 4,4′DDE, 0.14 µM for methoxychlor and 25 M for imidazole. GST activities were spectrophotometrically measured. SDS-PAGE and isoelectric focusing (IEF) were used to separate the different GST isoforms in control and treated animals, followed by Western blotting performed with anti-alpha, anti-mu and anti-pi GST anti-sera. In the hepatopancreas, GST-CDNB activities were always two to five-fold lower than in the gills where the activities were significantly increased after exposure to 4,4′DDE (ca. 1.6-fold) and to methoxychlor (ca. 1.3-fold) compared to the controls (ca. 3 µmol min<sup>−1</sup> mg−1protein) whereas they remained unchanged after treatment with imidazole. When using glutathione S-transferase anti-alpha, anti-mu and anti-pi anti-sera, a single 26 kDa polypeptide was observed in the hepatopancreas and in the gills in all the tested conditions. Five GST subunits were observed after IEF showing greater immuno-reactivity with the anti-pi mammalian class antiserum than with the anti-alpha or anti-mu mammalian anti-sera. One isoform of p<i>I</i> 5.77 was particularly induced by 4,4′DDE and methoxychlor; it was recognized by the three anti-sera tested and seemed to be more efficient in the gills than in the hepatopancreas. 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