{"refrec":{"BRefID":64179,"RR":"<b>Wang, J.; Seliskar, D.M.; Gallagher, J.L.</b> (2004). Plant regeneration via somatic embryogenesis in the brackish wetland monocot <i>Scirpus robustus</i>. <i>Aquat. Bot. 79(2)</i>: 163-174. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2004.02.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2004.02.003</a>","BEntID":60672,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Bot. 79(2)</i>: 163-174. <a href=\"https://dx.doi.org/10.1016/j.aquabot.2004.02.003\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquabot.2004.02.003</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Wang, J.; Seliskar, D.M.; Gallagher, J.L.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wang, J.; Seliskar, D.M.; Gallagher, J.L.","Englishabstract":"A tissue culture and plant regeneration protocol for salt marsh bulrush, <i>Scirpus robustus</i>, was developed. Callus was induced from seedling mesocotyl on Murashige and Skoog (MS) medium plus 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). Thirty-one percent of the calli became embryogenic upon transfer to MS medium supplemented with 0.25 mg/l 2,4-D. Limited plant regeneration via somatic embryogenesis was obtained after transferring the embryogenic callus to MS medium without growth regulators (MS0). The addition of 6-benzylaminopurine (BA) to the medium effectively increased the number of regenerated shoots and more shoots were regenerated on 3 mg/l BA medium (HBA) than on 0.1 mg/l BA medium (LBA). Transfer of the plants regenerated from BA-supplemented medium to MS0 medium further enhanced the development of the plants, which subsequently grew vigorously in potting soil in the greenhouse. Having a tissue culture and regeneration protocol for <i>S. robustus</i> makes it possible to take advantage of somaclonal variation for selecting lines of this species with characteristics desirable for wetland creation and restoration.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Plant regeneration via somatic embryogenesis in the brackish wetland monocot <i>Scirpus robustus</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-06 01:32:11.369620","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Scirpus robustus; tissue culture; somatic embryogenesis; regeneration;restoration","OtherDescriptors":"Scirpus robustus; Tissue culture; Somatic embryogenesis; Regeneration; Restoration","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2020-10-02","DateCreate":"2004-07-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000222192100005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquabot.2004.02.003"},"refs":null,"anarec":{"AnaID":64179,"PubliDate":2004,"Pagination":"163-174","XtraPublOfAnaID":null,"ISBN":null,"Volume":"79","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42195,"SerRR":"Aquatic Botany. 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