{"refrec":{"BRefID":65017,"RR":"<b>Conder, J.M.; La Point, T.W.; Bowen, A.T.</b> (2004). Preliminary kinetics and metabolism of 2,4,6-trinitrotoluene and its reduced metabolites in an aquatic oligochaete. <i>Aquat. Toxicol. 69(3)</i>: 199-213. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2004.04.013\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2004.04.013</a>","BEntID":61490,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquat. Toxicol. 69(3)</i>: 199-213. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2004.04.013\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2004.04.013</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Conder, J.M.; La Point, T.W.; Bowen, A.T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Conder, J.M.; La Point, T.W.; Bowen, A.T.","Englishabstract":"We examined the toxicokinetics and metabolism of 2,4,6-trinitirotoluene (TNT) and four of its major reduced metabolites (2-amino-4,6-dinitrotoluene (2ADNT), 4-amino-2,6-dinitrotoluene (4ADNT), and 2,4-diamino-6-nitrotoluene (2,4DANT)) in the freshwater, aquatic oligochaete <i>Tubifex tubifex</i> exposed to spiked, reconstituted water. In uptake experiments with each compound, steady state concentrations were reached within 1 h, and all absorbed compounds were completely eliminated in 0-3 h. The appearance of 2ADNT and 4ADNT (from metabolism of absorbed TNT) was five times slower, reaching 95% of steady state in 14.2-16.1 h. Approximately, 82% of absorbed TNT was metabolized to ADNTs; metabolism to 4ADNT was favored over 2ADNT by a factor of 3. No further metabolism of ADNTs to DANTs was detected. After a loss of 29-50% of metabolically-generated ADNTs during the first hour of the TNT depuration experiment, <i>Tubifex</i> ADNT concentrations remained constant throughout the 53 h depuration period. This suggests differences between the toxicokinetics of absorbed ADNTs and the toxicokinetics of metabolically-generated ADNTs. Experiments using radiolabeled (<sup>14</sup>C) TNT revealed that only 15-47% of <sup>14</sup>C-TNT equivalents were identified as TNT, 2ADNT, or 4ADNT, indicating significant metabolism and/or binding to biomolecules. Of unidentified <sup>14</sup>C-TNT equivalents, 28-38% remained unextractable. Both unextractable and extractable unidentified substances increased throughout the 54 h <sup>14</sup>C-TNT uptake experiment. The unidentified portions of the radiolabel were not eliminated during a 53-h depuration experiment. Bioconcentration factors (BCFs) for HPLC-detectable compounds were found to be linearly related to log K<sub>OW </sub>(r<sup>2</sup> = 0.9963). BCFs for 2ADNT, 4ADNT, and 2,4DANT were 10.22, 12.41, and 2.75, respectively. The BCF for TNT was 12.25, based on a molar sum of total TNT and its metabolites (∑TNT + 2ADNT + 4ADNT), and 2.53 based on TNT only. Compound hydrophobicity predicted the toxicokinetics and bioconcentration of compounds absorbed from water, however, the large discrepancy between the toxicokinetics of absorbed versus metabolically-generated ADNTs and the bioconcentration and toxicokinetics of the unidentified substances warrant further investigation","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Preliminary kinetics and metabolism of 2,4,6-trinitrotoluene and its reduced metabolites in an aquatic oligochaete","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:05.664933","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"elimination; nitroaromatics; explosives; bioconcentration; Tubifextubifex; biotransformation","OtherDescriptors":"Elimination; Nitroaromatics; Explosives; Bioconcentration; Tubifex tubifex; Biotransformation","Notes":null,"AnaPub":2004,"MonPub":null,"DateUpdate":"2020-10-05","DateCreate":"2004-08-16","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000223285400001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2004.04.013"},"refs":null,"anarec":{"AnaID":65017,"PubliDate":2004,"Pagination":"199-213","XtraPublOfAnaID":null,"ISBN":null,"Volume":"69","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. 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