{"refrec":{"BRefID":66086,"RR":"<b>Fidalgo, J.P.; Cid, A.; Abalde, J.; Herrero, C.</b> (1995). Culture of the marine diatom <i>Phaeodactylum tricornutum</i> with different nitrogen sources: growth, nutrient conversion and biochemical composition. <i>Cah. Biol. Mar. 36(3)</i>: 165-173","BEntID":62536,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Cah. Biol. Mar. 36(3)</i>: 165-173","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Fidalgo, J.P.; Cid, A.; Abalde, J.; Herrero, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Fidalgo, J.P. <i>et al.</i>","Englishabstract":"Cultures of the marine diatom <i>Phaeodactylum tricornutum</i> were grown in nitrate, nitrite, ammonia or urea media. Nitrogen transformation efficiencies were close to I0O% in cultures with nitrate, nitrite or urea, with productivities in dry biomass between 2.2 g.1 super(-1) (urea) and 2.8 g.1 super(-1) (nitrate). However growth in ammonia cultures ceased due to a drop in pH of the medium. The biochemical profile was more affected by the growth phase than by the N source. Cellular contents of chlorophylle a and c decreased and the chlorophyll alc ratio increased when cultures entered the stationary phase. Protein and RNA were higher during exponentiel growth (about 30% and 8% ash free dry weight (AFDW), respectively), but carbohydrates increased up to 220% in the stationary phase. Although significant differences were observed in cellular contents, the biochemical composition (as % AFDW) of the nitrate, nitrite and urea-grown cells was very similar. The highest total fatty acids and polyunsaturated fatty acids (PUFAS) contents in the stationary phase were obtained in the urea-grown cells, with an eicosapentaenoic acid (EPA) content of 26.8 mg.g super()-1 of dry biomass.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Culture of the marine diatom <i>Phaeodactylum tricornutum</i> with different nitrogen sources: growth, nutrient conversion and biochemical composition","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1995,"MonPub":null,"DateUpdate":"2016-03-11","DateCreate":"2004-09-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1995TV51100001","VABBcode":null,"OpenAcc":1},"refs":null,"anarec":{"AnaID":66086,"PubliDate":1995,"Pagination":"165-173","XtraPublOfAnaID":null,"ISBN":null,"Volume":"36","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42348,"SerRR":"Cahiers de Biologie Marine. 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