{"refrec":{"BRefID":68491,"RR":"<b>Tsoi, S.C.M.; Ewart, K.V.; Penny, S.; Melville, K.; Liebscher, R.S.; Brown, L.L.; Douglas, S.E.</b> (2004). Identification of immune-relevant genes from Atlantic salmon using suppression subtractive hybridization. <i>Mar. Biotechnol. 6(3)</i>: 199-214","BEntID":64712,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 6(3)</i>: 199-214","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tsoi, S.C.M.; Ewart, K.V.; Penny, S.; Melville, K.; Liebscher, R.S.; Brown, L.L.; Douglas, S.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tsoi, S.C.M. <i>et al.</i>","Englishabstract":"In order to probe the interaction between an invading microorganism and its host, we have investigated differential gene expression in Atlantic salmon (<i>Salmo salar</i>) experimentally infected with the pathogen <i>Aeromonas salmonicida</i>, the causative agent of furunculosis. Subtractive cDNA libraries were constructed by suppression subtractive hybridization (SSH) from 3 immune-relevant tissues at 2 time points during the infection process. Both forward- and reverse-subtracted libraries were generated, and approximately 200 clones were sequenced from each library, giving a total of 1778 expressed sequence tags (ESTs), which were annotated according to functional categories and deposited in GenBank (BQ035314-BQ037059). Numerous genes involved in signal transduction, innate immunity, and other processes have been uncovered in the subtractive libraries. These include known acute-phase reactants, along with more novel genes encoding proteins such as tachylectin, hepcidin, precerebellin-like protein, O-methyltransferase, a putative saxitoxin-binding protein, and others. 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