{"refrec":{"BRefID":70659,"RR":"Gülden, M.; Mörchel, S.; Seibert, H. (2005). Comparison of mammalian and fish cell line cytotoxicity: impact of endpoint and exposure duration. Aquat. Toxicol. 71(3): 229-236. https://dx.doi.org/10.1016/j.aquatox.2004.11.006","BEntID":66717,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Aquat. Toxicol. 71(3): 229-236. https://dx.doi.org/10.1016/j.aquatox.2004.11.006","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Gülden, M.; Mörchel, S.; Seibert, H.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gülden, M.; Mörchel, S.; Seibert, H.","Englishabstract":"Comparisons of acute toxic concentrations of chemicals to fish in vivo and cytotoxic concentrations to fish cell lines in vitro reveal rather good correlations of the toxic potencies in vitro and in vivo, but a clearly lower sensitivity of the fish cells. To examine whether the low sensitivity is specific for fish cells, cytotoxic potencies of reference chemicals from the Multicenter Evaluation of In Vitro Cytotoxicity program (MEIC) reported for the fish cell lines R1 and RTG-2 were compared with those obtained with the mouse Balb/c 3T3 cell line. Cytotoxic potencies (EC50 values) for MEIC reference chemicals were determined with exponentially growing Balb/c 3T3 cells using three different test protocols. To assess both endpoints, cell proliferation and cell survival, EC50 values were measured for the decrease in final cell protein after 24 and 72 h of exposure and for the reduction of cell protein increase during 24 h of exposure. EC50 values obtained with the fish cell lines R1 and RTG-2 using cell survival as endpoint were taken from the MEIC data base. The comparison of cytotoxic potencies shows that, in general, the fish cell lines and the mammalian cell line are almost equally sensitive towards the cytotoxic action of chemicals. The mammalian cell line assay, however, becomes considerably more sensitive, by factors of 3.4-8.5, than the fish cell line assays, if cell growth instead of cell survival is used as endpoint. It is concluded, that cell proliferation might be a better endpoint than cell survival and that mammalian cell lines might be suited to assess fish acute toxicity.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Comparison of mammalian and fish cell line cytotoxicity: impact of endpoint and exposure duration","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-14 01:31:10.734534","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"cytotoxic potency; MEIC; fish cell lines; Balb/c 3T3 cells; in vitrotoxicity testing","OtherDescriptors":"Cytotoxic potency; MEIC; Fish cell lines; Balb/c 3T3 cells; In vitro toxicity testing","Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2020-10-06","DateCreate":"2005-02-15","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000226987200003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2004.11.006"},"refs":null,"anarec":{"AnaID":70659,"PubliDate":2005,"Pagination":"229-236","XtraPublOfAnaID":null,"ISBN":null,"Volume":"71","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":229,"Pages":8,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"University Medical School Schleswig-Holstein","Email":"guelden@toxi.uni-kiel.de","Line2":"Campus Kiel, Brunswiker Str. 10, D-24105 Kiel","EnvName":"Germany"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Gülden","Firstname":"Michael","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":66717,"AutID":51388,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Mörchel","Firstname":"Sabine","Initials":"S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":66717,"AutID":51389,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Seibert","Firstname":"Hasso","Initials":"H.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":66717,"AutID":51390,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":351989,"LibID":36,"BRefID":70659,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"351989","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site en mag op geen enkele manier verder worden verspreid.