{"refrec":{"BRefID":71011,"RR":"<b>Dooms, S.; Deloof, D.; Dierckens, K.; Bossier, P.; Sorgeloos, P.</b> (2005). Genetic characterisation of commercially important <i>Brachionus</i> strains, <b><i>in</i></b>: Mees, J. <i>et al.</i> <i>VLIZ Young Scientists' Day, Brugge, Belgium 25 February 2005: book of abstracts. VLIZ Special Publication,</i> 20: pp. 33","BEntID":67064,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Mees, J. <i>et al.</i> <i>VLIZ Young Scientists' Day, Brugge, Belgium 25 February 2005: book of abstracts. VLIZ Special Publication,</i> 20: pp. 33","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Dooms, S.; Deloof, D.; Dierckens, K.; Bossier, P.; Sorgeloos, P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Dooms, S. <i>et al.</i>","Englishabstract":"The development of the mass production of high quality fingerlings of marine fish species in Europe was made possible by improvements in the techniques for producing and utilizing live food: rotifers of the <i>Brachionus plicatilis</i> species complex. In the past all commercially used rotifer strains were named <i>Brachionus plicatilis</i> (L-type) or <i>Brachionus rotundiformis</i> (S-type). But recent studies of natural <i>Brachionus</i> populations, based on the mitochondrial COI and genomic ITS1 molecular markers, revealed the existence of at least 9 biotypes of which recently 3 were (re)described as species: <i>B. plicatilis, B. rotundiformis, B. ibericus</i> (Gómez et al., 1995, 1996, 2002; Ciros-Pérez et al., 2001). This rotifer production is still the biggest problem for the fingerling production: the mass culture of these rotifers is very unpredictable. Periods with total mortality or reduced reproduction (‘crashes’) regularly occur. To get an idea of the genetic diversity of commercially used <i>Brachionus</i> strains, samples of hatcheries were analysed using the mitochondrial 16SrDNA molecular marker: polymorphisms are detected by the SSCP (Single Strand Conformation Polymorphism) and DGGE (Denaturing Gradient Gel Electrophoresis) technique  and by DNA sequencing. Our findings confirm the hypothesis of the existence of a <i>Brachionus plicatilis</i> species complex: so far 16 haplotypes were detected. Very few commercial hatcheries (not a single European hatchery!) massculture the true <i>Brachionus plicatilis</i> s.s.","AbstractOtherLang":null,"BibLvlCode":"AMS","StandardTitle":"Genetic characterisation of commercially important <i>Brachionus</i> strains","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2022-04-12","DateCreate":"2005-02-24","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":{"AnaID":71011,"PubliDate":2005,"Pagination":"33","XtraPublOfAnaID":null,"ISBN":null,"Volume":"20","Issue":null,"BRefMon":70967,"BRefMonRR":"<b>Mees, J.; Seys, J. (Ed.)</b> (2005). 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