{"refrec":{"BRefID":713,"RR":"<b>Lohse, L.; Kloosterhuis, H.T.; Van Raaphorst, W.; Helder, W.</b> (1996). Denitrification rates as measured by the isotope pairing method and by the acetylene inhibition technique in continental shelf sediments of the North Sea. <i>Mar. Ecol. Prog. Ser. 132</i>: 169-179","BEntID":713,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mar. Ecol. Prog. Ser. 132</i>: 169-179","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Lohse, L.; Kloosterhuis, H.T.; Van Raaphorst, W.; Helder, W.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Lohse, L. <i>et al.</i>","Englishabstract":"Denitrification rates in continental shelf sediments of the North Sea were measured by the simultaneous application of the acetylene inhibition technique (AIT) and the isotope pairing method (IPM). Nitrification was the predominant source of nitrate in these sediments. Both techniques were able to detect coupled nitrification-denitrification activity. Denitrification rates measured by the AIT were only measurable during the first 60 min of incubation. Rates obtained by the AIT in this period ranged between 5.4 and 7.3 µmol N m<sup>-2</sup> h<sup>-1</sup>. Continued incubation of acetylene amended sediment cores resulted in decreasing denitrification rates, suggesting that accumulated nitrous oxide was consumed despite the presence of acetylene. Denitrification rates obtained by the application of the IPM ranged between 9.8 and 13.2 µmol N m<sup>-2</sup> h<sup>-1</sup>. Constant production of single-labelled (<sup>14</sup>N<sup>15</sup>N) relative to double-labelled (<sup>15</sup>N<sup>15</sup>N) dinitrogen indicated a homogeneous mixing of both isotopic nitrate species through the denitrification zone. The present results suggest that previously published data severely underestimated denitrification rates in North Sea continental shelf sediments. Concomitant measured fluxes of nitrate and ammonium across the sediment-water interface reveal that in these sediments most of the deposited nitrogen is returned to the water column as ammonium and nitrate.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Denitrification rates as measured by the isotope pairing method and by the acetylene inhibition technique in continental shelf sediments of the North Sea","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2025-07-02 08:33:14.980000","timezone_type":1,"timezone":"+00:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1996,"MonPub":null,"DateUpdate":"2019-08-22","DateCreate":"2000-08-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1996UD22300016","VABBcode":null,"OpenAcc":1},"refs":null,"anarec":{"AnaID":713,"PubliDate":1996,"Pagination":"169-179","XtraPublOfAnaID":null,"ISBN":null,"Volume":"132","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43354,"SerRR":"Marine Ecology Progress Series. Inter-Research: Oldendorf/Luhe.  ISSN 0171-8630; e-ISSN 1616-1599","StandardTitleSer":"Marine Ecology Progress Series","ISSN":"0171-8630","AbbrevSer":"Mar. Ecol. Prog. Ser.","StandardTitleMon":null,"StartPage":169,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"PO Box 59, 1790 AB Den Burg, Texel","Email":"lutz@nioz.nl","Line2":null,"EnvName":"Netherlands"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Lohse","Firstname":"Lutz","Initials":"L.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":713,"AutID":1587,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Kloosterhuis","Firstname":"Henderikus","Initials":"H.T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":713,"AutID":1588,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Van Raaphorst","Firstname":null,"Initials":"W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":713,"AutID":1388,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Helder","Firstname":"Willem","Initials":"W.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":713,"AutID":1590,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":335816,"LibID":36,"BRefID":713,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"335816","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Inter-Research","City":"Oldendorf/Luhe"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Ammonium compounds","ThestID":320,"Acronym":"ASFA","ThesTermPath":"Ammonium compounds"},{"ThesaurusTerm":"Sediment analysis","ThestID":7360,"Acronym":"ASFA","ThesTermPath":"Analysis > Sediment analysis"},{"ThesaurusTerm":"ANE, North Sea","ThestID":182108,"Acronym":"ASFA_GEOT","ThesTermPath":"ANE, North Sea"},{"ThesaurusTerm":"Nitrates","ThestID":5561,"Acronym":"ASFA","ThesTermPath":"Chemical compounds > Nitrogen compounds > Nitrates"},{"ThesaurusTerm":"Denitrification","ThestID":2200,"Acronym":"ASFA","ThesTermPath":"Chemical reactions > Denitrification"},{"ThesaurusTerm":"Sediment-water interface","ThestID":7395,"Acronym":"ASFA","ThesTermPath":"Interfaces > Sediment-water interface"},{"ThesaurusTerm":"Oxygen profiles","ThestID":5939,"Acronym":"ASFA","ThesTermPath":"Profiles > Vertical profiles > Oxygen profiles"},{"ThesaurusTerm":"Radioactive labelling","ThestID":6704,"Acronym":"ASFA","ThesTermPath":"Radioactive labelling"},{"ThesaurusTerm":"Continental shelves","ThestID":1838,"Acronym":"ASFA","ThesTermPath":"Topographic features > Submarine features > Continental shelves"},{"ThesaurusTerm":"Pore water","ThestID":6453,"Acronym":"ASFA","ThesTermPath":"Water > Pore water"},{"ThesaurusTerm":"Eutrophic lakes","ThestID":2930,"Acronym":"ASFA","ThesTermPath":"Water bodies > Inland waters > Lakes > Eutrophic lakes"}],"thestermsASFA":[{"ThesaurusTerm":"Ammonium compounds"},{"ThesaurusTerm":"ANE, North Sea"},{"ThesaurusTerm":"Continental shelves"},{"ThesaurusTerm":"Denitrification"},{"ThesaurusTerm":"Eutrophic lakes"},{"ThesaurusTerm":"Nitrates"},{"ThesaurusTerm":"Oxygen profiles"},{"ThesaurusTerm":"Pore water"},{"ThesaurusTerm":"Radioactive labelling"},{"ThesaurusTerm":"Sediment analysis"},{"ThesaurusTerm":"Sediment-water interface"}],"taxtermsASFA":null,"geotermsASFA":[{"GeoTerm":"North Sea"}],"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":713,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":713,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":[{"ThesaurusTerm":"Ammonium compounds","ThestID":320,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"ANE, North Sea","ThestID":182108,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Continental shelves","ThestID":1838,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Denitrification","ThestID":2200,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Eutrophic lakes","ThestID":2930,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Nitrates","ThestID":5561,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Oxygen profiles","ThestID":5939,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Pore water","ThestID":6453,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Radioactive labelling","ThestID":6704,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sediment analysis","ThestID":7360,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sediment-water interface","ThestID":7395,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":null,"geoterms":[{"GeoTerm":"North Sea","GeoObjectID":2350,"GeotID":390}],"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Ammonium compounds","DutchTerm":"Ammoniumcomponenten","ThestID":320,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"ANE, North Sea","DutchTerm":null,"ThestID":182108,"Acronym":"ASFA_GEOT","ThesTypID":34,"ThesType":"ASFA Geoterms"},{"ThesaurusTerm":"Continental shelves","DutchTerm":"Continentaal plat","ThestID":1838,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Denitrification","DutchTerm":"Denitrificatie","ThestID":2200,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Eutrophic lakes","DutchTerm":null,"ThestID":2930,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Nitrates","DutchTerm":"Nitraten","ThestID":5561,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Oxygen profiles","DutchTerm":"Zuurstofprofiel","ThestID":5939,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Pore water","DutchTerm":"Poriënwater","ThestID":6453,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Radioactive labelling","DutchTerm":"Radioactieve labelling","ThestID":6704,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sediment analysis","DutchTerm":"Sedimentanalyse","ThestID":7360,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Sediment-water interface","DutchTerm":"Sediment-water interface","ThestID":7395,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":null,"geotermsFRIS":[{"GeoTerm":"North Sea","DutchTerm":"Noordzee"}],"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Appeltans, Ward, W.","newSesDate":{"date":"2000-08-24 19:15:10.000000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2019-08-22 08:36:28.553000","timezone_type":3,"timezone":"Europe/Brussels"}}}