{"refrec":{"BRefID":72867,"RR":"<b>Tang, J.Y.M.; Anderson, D.M.; Au, D.W.T.</b> (2005). Hydrogen peroxide is not the cause of fish kills associated with <i>Chattonella marina</i>: cytological and physiological evidence. <i>Aquat. Toxicol. 72(4)</i>: 351-360","BEntID":68795,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 72(4)</i>: 351-360","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Tang, J.Y.M.; Anderson, D.M.; Au, D.W.T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Tang, J.Y.M.; Anderson, D.M.; Au, D.W.T.","Englishabstract":"Chattonella marina, a harmful algal bloom (HAB) causative species, was used to study the mortality, physiology, and pathology of a marine stenohaline fish, goldlined seabream exposed to the toxic alga. The median lethal time (LT<sub>50</sub>) was 3h upon exposure to 8000cells/ml of <i>C. marina</i>. Significant induction of filamental chloride cells (CCs) [i.e. increases in CC fractional area and in the volume density of CCs], concomitant with significant reduction of blood osmolality, were found in <i>C. marina</i> treated fish. To verify whether the toxicity of <i>C. marina</i> was mediated through oxidative stress, a hydrogen peroxide exposure experiment was carried out and the toxicity as well as cytological and physiological changes were compared with the <i>C. marina</i> treatment. Hydrogen peroxide at a concentration of 500 µM H<sub>2</sub>)O<sub>2</sub>, (i.e. 25 times higher than that produced by 8000cells/ml of <i>C. marina</i> (20 µM H<sub>2</sub>O<sub>2</sub>) was unable to induce similar CC alterations and osmoregulatory impairment in fish as observed in the <i>C. marina</i> treatment. Non-specific membrane damage such as severe loss of microvilli projections on the CC apical opening and rupture of epithelial membranes in the lamellae were observed. The LT<sub>50</sub> was 6h, two times longer than that with 8000cells/ml of <i>C. marina</i>. Based on the cytological and physiological evidence and toxicity data, the mechanism by which <i>C. marina</i> kills fish appears to be very different from that caused by H<sub>2</sub>O<sub>2</sub>/ROS. Osmoregulatory distress is the major cause of fish death upon exposure to <i>C. marina</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Hydrogen peroxide is not the cause of fish kills associated with <i>Chattonella marina</i>: cytological and physiological evidence","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2005-05-23","DateCreate":"2005-05-18","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":72867,"PubliDate":2005,"Pagination":"351-360","XtraPublOfAnaID":null,"ISBN":null,"Volume":"72","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. 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