{"refrec":{"BRefID":73133,"RR":"<b>Liu, S.-Y.V.; Dai, C.-F.; Fan, T.-Y.; Yu, H.-T.</b> (2005). Cloning and characterization of microsatellite Loci in a gorgonian coral, <i>Junceella juncea</i> (Anthozoa; Octocorallia; Ellisellidae) and its application in clonal genotyping. <i>Mar. Biotechnol. 7(1)</i>: 26-32. <a href=\"https://dx.doi.org/10.1007/s10126-004-0015-2\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0015-2</a>","BEntID":69035,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 7(1)</i>: 26-32. <a href=\"https://dx.doi.org/10.1007/s10126-004-0015-2\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0015-2</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Liu, S.-Y.V.; Dai, C.-F.; Fan, T.-Y.; Yu, H.-T.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Liu, S.-Y.V. <i>et al.</i>","Englishabstract":"We attempted to isolate microsatellites from a <i>Symbiodinium-free</i> gorgonian octocoral, <i>Junceella juncea</i>, using two methods, partial genomic library screening and enrichment. Among the 3856 clones screened by the partial library method, 10 possibly positive signals were found, and 3 of them could be used to design primers and amplified consistently. In contrast, only one locus isolated by the enrichment method gave reliable amplification and was useful. The results indicate that microsatellites are rare in <i>Junceella juncea</i>, as reported for other cnidarians. Overall, we obtained 4 polymorphic loci to test the feasibility in investigating clonal structure of <i>J. juncea</i>. A total of 40 multilocus genotypes were found among 152 colonies, and the number of genotypes (clones) identified at 7 reefs ranged from 2 to 16. The results of a nonmetric multidimensional scaling analysis indicated the recruitment of <i>J. juncea</i> populations mainly comes from self-retention. These novel microsatellite loci will provide a useful tool to study clonal structure and population genetics for <i>J. juncea</i> in the future.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Cloning and characterization of microsatellite Loci in a gorgonian coral, <i>Junceella juncea</i> (Anthozoa; Octocorallia; Ellisellidae) and its application in clonal genotyping","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-15 01:32:15.265896","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Junceella; microsatellite; clonal structure; coral","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2020-10-06","DateCreate":"2005-06-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000229540200004","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-004-0015-2"},"refs":null,"anarec":{"AnaID":73133,"PubliDate":2005,"Pagination":"26-32","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. Springer-Verlag: New York.  ISSN 1436-2228; e-ISSN 1436-2236","StandardTitleSer":"Marine Biotechnology","ISSN":"1436-2228","AbbrevSer":"Mar. Biotechnol.","StandardTitleMon":null,"StartPage":26,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"ROC 10617 Taipei","Email":null,"Line2":null,"EnvName":"Taiwan, Province of China"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Liu","Firstname":"Shang-Yin Vanson","Initials":"S.-Y.V.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":69035,"AutID":52978,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Dai","Firstname":"Chang-Feng","Initials":"C.-F.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":69035,"AutID":7744,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Fan","Firstname":"Tung-Yung","Initials":"T.-Y.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":69035,"AutID":52979,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Yu","Firstname":"Hon-Tsen","Initials":"H.-T.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":69035,"AutID":52980,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":352113,"LibID":36,"BRefID":73133,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"352113","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer-Verlag","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Coral","ThestID":1881,"Acronym":"ASFA","ThesTermPath":"Animal products > Coral"},{"ThesaurusTerm":"Microsatellites","ThestID":60011,"Acronym":"CSA","ThesTermPath":"Microsatellites"},{"ThesaurusTerm":"Microsatellites","ThestID":154085,"Acronym":"CAB","ThesTermPath":"Nucleic compounds > Nucleic acids > Dna > Satellite dna > Microsatellites"}],"thestermsASFA":[{"ThesaurusTerm":"Coral"},{"ThesaurusTerm":"Microsatellites"},{"ThesaurusTerm":"Microsatellites"}],"taxtermsASFA":[{"TaxTerm":"Junceella juncea"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":69035,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":69035,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s10126-004-0015-2","externalID":"10.1007/s10126-004-0015-2","URLTypeCode":"DOI","URLID":3848,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":[{"ThesaurusTerm":"Coral","ThestID":1881,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Microsatellites","ThestID":60011,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Microsatellites","ThestID":154085,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxterms":[{"TaxTerm":"Junceella juncea","AphiaID":212767,"TaxtID":34947}],"geoterms":null,"othterms":[{"OtherTerm":"Clonal structure","OthtID":13153}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Coral","DutchTerm":"Koraal","ThestID":1881,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Microsatellites","DutchTerm":null,"ThestID":60011,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Microsatellites","DutchTerm":null,"ThestID":154085,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"}],"taxtermsFRIS":[{"TaxTerm":"Junceella juncea","DutchTerm":null,"AphiaID":212767,"TaxtID":34947}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Clonal structure","DutchTerm":null,"OthtID":13153}],"resmessage":"","complete":1,"sessions":{"newSesName":"VLIZ2000\\stevenc","newSesDate":{"date":"2005-06-07 08:41:33.747000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Bouchti, Zohra, Z.","updSesDate":{"date":"2020-10-06 07:02:16.303000","timezone_type":3,"timezone":"Europe/Brussels"}}}