{"refrec":{"BRefID":73137,"RR":"<b>Jarasrassamee, B.; Supungul, P.; Panyim, S.; Klinbunga, S.; Rimphanichayakit, V.; Tassanakajon, A.</b> (2005). Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (<i>Penaeus monodon</i>). <i>Mar. Biotechnol. 7(1)</i>: 46-52. <a href=\"https://dx.doi.org/10.1007/s10126-004-0100-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0100-6</a>","BEntID":69039,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Mar. Biotechnol. 7(1)</i>: 46-52. <a href=\"https://dx.doi.org/10.1007/s10126-004-0100-6\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0100-6</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Jarasrassamee, B.; Supungul, P.; Panyim, S.; Klinbunga, S.; Rimphanichayakit, V.; Tassanakajon, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Jarasrassamee, B. <i>et al.</i>","Englishabstract":"Four full-length complementary DNAs of Kazal-type serine proteinase inhibitors (SPIs) were identified from hemocyte cDNA libraries of the black tiger shrimp <i>Penaeus monodon</i> and recognized as having 4 (SPIPm1) and 5 (SPIPm2, SPIPm3, and SPIPm4) Kazal domains. SPIPm2 encoding a complete 5-Kazal-domain inhibitor was expressed in the <i>Escherichia coli</i> system. Inhibitory activity of crude proteins against various serine proteases was tested using SPI activity gelatin with sodium dodecylsulfate polyacrylamide gel electrophoresis and inhibitory spectrum assays. A 32-kDa recombinant protein (rSPIPm2) showed inhibitory activity against trypsin, chymotrypsin, and subtilisin, but not elastase. Concordantly, inhibitory spectrum assays showed that crude rSPIPm2 strongly inhibited trypsin (89%) and chymotrypsin (70%), but less effectively inhibited subtilisin (8%), and did not inhibit elastase activity. Northern blot analysis of hemocyte total RNA showed 2 SPI transcripts of 1.6 and 1.7 kb in size. Tissue-specific expression using reverse transcriptase polymerase chain reaction suggests that SPIPm2 is exclusively expressed in hemocytes of <i>P. monodon</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Recombinant expression and characterization of five-domain Kazal-type serine proteinase inhibitor of black tiger shrimp (<i>Penaeus monodon</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-13 01:31:06.743149","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Penaeus monodon; black tiger shrimp; serine proteinase inhibitors; Kazaldomain; hemocytes","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2020-10-06","DateCreate":"2005-06-07","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000229540200007","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-004-0100-6"},"refs":null,"anarec":{"AnaID":73137,"PubliDate":2005,"Pagination":"46-52","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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