{"refrec":{"BRefID":7327,"RR":"<b>Gharrett, A.J.; Gray, A.K.; Heifetz, J.</b> (2001). Identification of rockfish (<i>Sebastes</i> spp.) by restriction site analysis of the mitochondrial ND-3/ND-4 and 12S/16S rRNA gene regions. <i>Fish. Bull. 99(1)</i>: 49-62","BEntID":7327,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Fish. Bull. 99(1)</i>: 49-62","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Gharrett, A.J.; Gray, A.K.; Heifetz, J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gharrett, A.J.; Gray, A.K.; Heifetz, J.","Englishabstract":"Species-specific restriction variation in the 12S/16S rRNA ND-3/ND-4 mtDNA regions was used to distinguish among 15 rockfish species of the genus <i>Sebastes</i> common to the waters of Alaska. Intraspecific variation exhibited by eight of the species (based on five individuals of each species) did not obscure the interspecific variation, except possibly between <i>S. zacentrus</i> and <i>S. variegatus</i>. Intra-specific nucleotide diversity averaged 0.0024 substitutions per nucleotide, whereas interspecific nucleotide divergence averaged 0.0249. In contrast, the average nucleotide divergences between <i>Sebastes</i> and two other scorpaenid species,<i>Helicolenus hilgendorfi</i> and <i>Sebastolobus alascanus</i>, were 0.0805 and 0.1073, respectively. Cladistic and phenetic analyses supported some, but not all, of the subgenera assignments of <i>Sebastes</i>. A scheme for distinguishing among the species studied was presented. Restriction sites of 10 restriction endonucleases were mapped in the two PCR-amplified mtDNA regions by using double digests. In all, we detected 153 sites corresponding to 640 (13.5%) of the 4815 nucleotides in the two regions combined. The ND-3/ND-4 region exhibited substantially more intraspecific, interspecific, and intergeneric variation than the 12S/16S rRNA region.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Identification of rockfish (<i>Sebastes</i> spp.) by restriction site analysis of the mitochondrial ND-3/ND-4 and 12S/16S rRNA gene regions","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2001,"MonPub":null,"DateUpdate":"2001-12-11","DateCreate":"2001-04-03","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000167063300005","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":7327,"PubliDate":2001,"Pagination":"49-62","XtraPublOfAnaID":null,"ISBN":null,"Volume":"99","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44319,"SerRR":"Fishery Bulletin. US Government Printing Office: Washington, D.C..  ISSN 0090-0656; e-ISSN 1937-4518","StandardTitleSer":"Fishery Bulletin","ISSN":"0090-0656","AbbrevSer":"Fish. Bull.","StandardTitleMon":null,"StartPage":49,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":"Nummering verandert vanaf 1963, issuenummer wordt aangepast en men gaat over na vier nummers per jaar. Volumenummer wordt aangepast. In het begin bestond het nr. van het Bulletin uit een extract van het bulletin van het US Bureau of fisheries."},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Gharrett","Firstname":"Anthony","Initials":"A.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":7327,"AutID":11485,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gray","Firstname":"Andrew","Initials":"A.K.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":7327,"AutID":11486,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Heifetz","Firstname":"Jonathan","Initials":"J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":7327,"AutID":11487,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"US Government Printing Office","City":"Washington, D.C."}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":7327,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2001-04-03 10:11:40.337000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Haspeslagh, Jan, J.","updSesDate":{"date":"2001-12-11 11:16:22.383000","timezone_type":3,"timezone":"Europe/Brussels"}}}