{"refrec":{"BRefID":75194,"RR":"<b>Veuger, B.; Middelburg, J.J.; Boschker, H.T.S.; Houtekamer, M.</b> (2005). Analysis of <sup>15</sup>N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria. <i>Limnol. Oceanogr., Methods 3</i>: 230-240","BEntID":70879,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Limnol. Oceanogr., Methods 3</i>: 230-240","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Veuger, B.; Middelburg, J.J.; Boschker, H.T.S.; Houtekamer, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Veuger, B. <i>et al.</i>","Englishabstract":"The quantitative contribution of bacteria to total microbial uptake of nitrogenous substrates is an aspect of the aquatic nitrogen cycle that is still largely unclear, mainly because existing methods are generally inadequate. We investigated the feasibility of measuring <sup>15</sup>N incorporation into bacterial D-amino acids by gas chromatography- combustion-isotope ratio mass spectrometry (GC-c-IRMS) and the potential of this method as a new tool for quantification of <sup>15</sup>N uptake by bacteria. The presented method allowed analysis of <sup>15</sup>N incorporation into various hydrolysable amino acids (HAAs), including the bacterial biomarker D-alanine (D-Ala), at trace levels. The potential of the method was tested in a <sup>15</sup>N labeling experiment in which sediment slurries were incubated with <sup>15</sup>NH<sub>4</sub><sup>+</sup> and a <sup>15</sup>N-labeled amino acid mixture. <sup>15</sup>N incorporation into D-Ala was used to calculate total bacterial <sup>15</sup>N uptake while comparison of <sup>15</sup>N incorporation into D-Ala versus L-Ala provided a direct measure for the relative contributions of bacteria versus algae to the total microbial <sup>15</sup>N uptake. Subsequently, it was also possible to calculate <sup>15</sup>N uptake by algae. Results for the test experiment showed that bacteria accounted for 38% of total <sup>15</sup>NH<sub>4</sub><sup>+</sup> uptake and dominated uptake of the <sup>15</sup>N-amino acid mixture (90%). Analysis of <sup>15</sup>N incorporation into other (non-biomarker) HAAs yielded useful additional information on the transformation of these HAAs during organic matter degradation. In conclusion, GC-c-IRMS analysis of D-Ala combined with <sup>15</sup>N labeling is a unique approach in aquatic sciences that provides a powerful new method for quantification of nitrogen flows through bacteria in natural microbial communities.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Analysis of <sup>15</sup>N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2005-09-05","DateCreate":"2005-08-04","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":1},"refs":null,"anarec":{"AnaID":75194,"PubliDate":2005,"Pagination":"230-240","XtraPublOfAnaID":null,"ISBN":null,"Volume":"3","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":75192,"SerRR":"Limnology and Oceanography: Methods. American Society of Limnology and Oceanography: Waco, Tex..  ISSN 1541-5856; e-ISSN 1541-5856","StandardTitleSer":"Limnology and Oceanography: Methods","ISSN":"1541-5856","AbbrevSer":"Limnol. Oceanogr., Methods","StandardTitleMon":null,"StartPage":230,"Pages":11,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Veuger","Firstname":null,"Initials":"B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":70879,"AutID":45603,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":8761,"InsID":null},{"AutName":"Middelburg","Firstname":null,"Initials":"J.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":70879,"AutID":54518,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0003-3601-9072","PersID":3477,"InsID":null},{"AutName":"Boschker","Firstname":"Henricus","Initials":"H.T.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":70879,"AutID":23337,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":"0000-0002-0947-7919","PersID":3476,"InsID":null},{"AutName":"Houtekamer","Firstname":"Marco","Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":70879,"AutID":54547,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":72163,"LibID":36,"BRefID":75194,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"72163","RFID":null,"PaidValue":null,"Medium":"Server","Description":"VLIZ Open Access","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Open access","URL":null,"ClassifID":53,"Classification":"Open access","ReqLink":null,"ClassifTypID":1,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":null,"InternalReq":0,"LoggedInReq":0,"Disclaimer":null,"DutchDisclaimer":null,"FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"American Society of Limnology and Oceanography","City":"Waco, Tex."}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":null,"geotermsASFA":null,"collections":[{"Collection":"NIOO-CEME collection","ShortName":"nioo-ceme collection"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"345":{"SpName":"NIOO-CEME collection","SpColID":345,"ParSpColID":null,"TopParID":null,"ShortName":"nioo-ceme collection","URLLocation":null,"LibID":null,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"nioo-ceme collection"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":70879,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":70879,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":null,"taxterms":null,"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2005-08-04 14:16:30.620000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Haspeslagh, Jan, J.","updSesDate":{"date":"2005-09-05 09:33:30.240000","timezone_type":3,"timezone":"Europe/Brussels"}}}