{"refrec":{"BRefID":7584,"RR":"<b>De Schamphelaere, K.A.C.; Janssen, C.R.<\/b> (2002). A biotic ligand model predicting acute copper toxicity for <i>Daphnia magna<\/i>: effects of calcium, magnesium, sodium, potassium and pH. <i>Environ. Sci. Technol. 36(1)<\/i>: 48-54. <a href=\"https:\/\/dx.doi.org\/10.1021\/es000253s\" target=\"_blank\">https:\/\/dx.doi.org\/10.1021\/es000253s<\/a>","BEntID":7584,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Environ. Sci. Technol. 36(1)<\/i>: 48-54. <a href=\"https:\/\/dx.doi.org\/10.1021\/es000253s\" target=\"_blank\">https:\/\/dx.doi.org\/10.1021\/es000253s<\/a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":1,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"De Schamphelaere, K.A.C.; Janssen, C.R.","OrigTitleTranslFlag":0,"Authorstringtrunc":"De Schamphelaere, K.A.C.; Janssen, C.R.","Englishabstract":"The extent to which Ca<sup>2+<\/sup>, Mg<sup>2+<\/sup>, Na<sup>+<\/sup>, K<sup>+<\/sup> ions and pH independently mitigate acute copper toxicity for the cladoceran <i>Daphnia magna<\/i> was examined. Higher activities of Ca<sup>2+<\/sup>, Mg<sup>2+<\/sup>, and Na<sup>+<\/sup> (but not K<sup>+<\/sup>) linearly increased the 48-h EC50 (as Cu<sup>2+<\/sup> activity), supporting the concept of competitive binding of these ions and copper ions to toxic action or transport sites at the organism\u2212water interface (e.g. fish gill, the biotic ligand). The increase of the EC50 (as Cu<sup>2+<\/sup> activity) with increasing H<sup>+<\/sup>, however, seemed to suggest cotoxicity of CuOH<sup>+<\/sup> rather than proton competition. Based on the biotic ligand model (BLM) concept, we developed a methodology to estimate stability constants for the binding of Cu<sup>2+<\/sup>, CuOH<sup>+<\/sup>, Ca<sup>2+<\/sup>, Mg<sup>2+<\/sup>, Na<sup>+<\/sup>, and H<sup>+<\/sup> to the biotic ligand, solely based on toxicity data. Following values were obtained:\u2009 log <i>K<\/i><sub>CuBL<\/sub> = 8.02, log <i>K<\/i><sub>CuOHBL<\/sub>= 7.45, log <i>K<\/i><sub>CaBL<\/sub> = 3.47, log <i>K<\/i><sub>MgBL<\/sub> = 3.58, log <i>K<\/i><sub>NaBL<\/sub> = 3.19, and log <i>K<\/i><sub>HBL<\/sub> \u223c 5.4. Further, we calculated that on average 39% of the biotic ligand sites need to be occupied by copper to induce a 50% acute effect for <i>D. magna<\/i> after 48 h of exposure. Using the estimated constants, a BLM was developed that can predict acute copper toxicity for <i>D. magna <\/i>as a function of water characteristics. The presented methodology can easily be applied for BLM development for other organisms and metals. After validation with laboratory and natural waters (including DOC), the developed model will support efforts to improve the ecological relevance of presently applied risk assessment procedures. 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American Chemical Society: Easton.  ISSN 0013-936X; e-ISSN 1520-5851","StandardTitleSer":"Environmental Science and Technology","ISSN":"0013-936X","AbbrevSer":"Environ. Sci. 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