{"refrec":{"BRefID":75851,"RR":"<b>Wadman, S.A.; Clark, K.J.; Hackett, P.B.</b> (2005). Fishing for answers with transposons. <i>Mar. Biotechnol. 7(3)</i>: 135-141","BEntID":71519,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(3)</i>: 135-141","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Wadman, S.A.; Clark, K.J.; Hackett, P.B.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wadman, S.A.; Clark, K.J.; Hackett, P.B.","Englishabstract":"Transposons are one means that nature has used to introduce new genetic material into chromosomes of organisms from every kingdom. They have been extensively used in prokaryotic and lower eukaryotic systems, but until recently there was no transposon that had significant activity in vertebrates. The Sleeping Beauty (SB) transposon system was developed  to direct the integration of precise DNA sequences into chromosomes. The SB system was derived from salmonid sequences that had been inactive for more than 10 million years. SB transposons have been used for two principle uses &#x2013; as a vector for transgenesis and as a method for introducing various trap vectors into (gene-trap) or in the neighborhood of (enhancer-trap) genes to identify their functions. Results of these studies show that SB-mediated transgenesis is more efficient than that by injection of simple plasmids and that expression of transgenesis is stable and reliable following passage through the germline.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Fishing for answers with transposons","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2005-09-05","DateCreate":"2005-08-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000230431000001","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":75851,"PubliDate":2005,"Pagination":"135-141","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. Springer-Verlag: New York.  ISSN 1436-2228; e-ISSN 1436-2236","StandardTitleSer":"Marine Biotechnology","ISSN":"1436-2228","AbbrevSer":"Mar. Biotechnol.","StandardTitleMon":null,"StartPage":135,"Pages":7,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Wadman","Firstname":"Shannon","Initials":"S.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":71519,"AutID":54916,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Clark","Firstname":"Karl","Initials":"K.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":71519,"AutID":54917,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Hackett","Firstname":"Perry","Initials":"P.B.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":71519,"AutID":54918,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":73138,"LibID":36,"BRefID":75851,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":1,"ShelfLocCode":"73138","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer-Verlag","City":"New York"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"Genes","ThestID":3589,"Acronym":"ASFA","ThesTermPath":"Cell constituents > Chromosomes > Genes"},{"ThesaurusTerm":"Fish","ThestID":3141,"Acronym":"ASFA","ThesTermPath":"Fauna > Aquatic organisms > Aquatic animals > Fish"},{"ThesaurusTerm":"Health and safety","ThestID":3928,"Acronym":"ASFA","ThesTermPath":"Health and safety"}],"thestermsASFA":[{"ThesaurusTerm":"Fish"},{"ThesaurusTerm":"Genes"},{"ThesaurusTerm":"Health and safety"}],"taxtermsASFA":null,"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":71519,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":71519,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":[{"ThesaurusTerm":"Fish","ThestID":3141,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Genes","ThestID":3589,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Health and safety","ThestID":3928,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":null,"geoterms":null,"othterms":[{"OtherTerm":"Enhancer trap","OthtID":13393},{"OtherTerm":"Gene trap","OthtID":13394},{"OtherTerm":"Transgenesis","OthtID":13396}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Fish","DutchTerm":"Vis","ThestID":3141,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Genes","DutchTerm":"Genen","ThestID":3589,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Health and safety","DutchTerm":"Gezondheid en Veiligheid","ThestID":3928,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":null,"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Enhancer trap","DutchTerm":null,"OthtID":13393},{"OtherTerm":"Gene trap","DutchTerm":null,"OthtID":13394},{"OtherTerm":"Transgenesis","DutchTerm":null,"OthtID":13396}],"resmessage":"","complete":1,"sessions":{"newSesName":"Chisala, Chilekwa, C.","newSesDate":{"date":"2005-08-25 12:31:39.150000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Haspeslagh, Jan, J.","updSesDate":{"date":"2005-09-05 09:33:30.240000","timezone_type":3,"timezone":"Europe/Brussels"}}}