{"refrec":{"BRefID":75915,"RR":"<b>Kanno, M.; Li, Q.; Kijima, A.</b> (2005). Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (<i>Stichopus japonicus</i>). <i>Mar. Biotechnol. 7(3)</i>: 179-183. <a href=\"https://dx.doi.org/10.1007/s10126-004-0006-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0006-3</a>","BEntID":71584,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(3)</i>: 179-183. <a href=\"https://dx.doi.org/10.1007/s10126-004-0006-3\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0006-3</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kanno, M.; Li, Q.; Kijima, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kanno, M.; Li, Q.; Kijima, A.","Englishabstract":"Twenty microsatellite markers were first developed from the Japanese sea cucumber <i>Stichopus japonicus</i> using an enrichment protocol. Of the 20 microsatellite loci, 19 loci were polymorphic in the population examined. At these polymorphic loci, the number of alleles per locus varied from 2 to 15, and the observed heterozygosities ranged from 0.03 to 0.97, which is considerably higher than those previously found for allozymes. 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