{"refrec":{"BRefID":75918,"RR":"<b>Wang, Y.; Xu, Z.; Pierce, J.C.; Guo, X.</b> (2005). Characterization of eastern oyster (<i>Crassostrea virginica</i> Gmelin) chromosomes by fluorescence in situ hybridization with bacteriophage P1 clones. <i>Mar. Biotechnol. 7(3)</i>: 207-214. <a href=\"https://dx.doi.org/10.1007/s10126-004-0051-y\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0051-y</a>","BEntID":71587,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(3)</i>: 207-214. <a href=\"https://dx.doi.org/10.1007/s10126-004-0051-y\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0051-y</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Wang, Y.; Xu, Z.; Pierce, J.C.; Guo, X.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Wang, Y. <i>et al.</i>","Englishabstract":"Chromosome identification is an essential step in genomic research, which so far has not been possible in oysters. We tested bacteriophage P1 clones for chromosomal identification in the eastern oyster <i>Crassostrea virginica</i>, using fluorescence in situ hybridization (FISH). P1 clones were labeled with digoxigenin-11-dUTP using nick translation. Hybridization was detected with fluorescein-isothiocyanate-labeled anti-digoxigenin antibodies and amplified with 2 layers of antibodies. Nine of the 21 P1 clones tested produced clear and consistent FISH signals when Cot-1 DNA was used as a blocking agent against repetitive sequences. Karyotypic analysis and cohybridization positively assigned the 9 P1 clones to 7 chromosomes. The remaining 3 chromosomes can be separated by size and arm ratio. Five of the 9 P1 clones were sequenced at both ends, providing sequence-tagged sites that can be used to integrate linkage and cytogenetic maps. One sequence is part of the bone morphogenetic protein type 1b receptor, a member of the transforming growth factor superfamily, and mapped to the telomeric region of the long arm of chromosome 2. This study shows that large-insert clones such as P1 are useful as chromosome-specific FISH probes and for gene mapping in oysters.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Characterization of eastern oyster (<i>Crassostrea virginica</i> Gmelin) chromosomes by fluorescence in situ hybridization with bacteriophage P1 clones","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-05 01:30:47.844395","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2005-09-05","DateCreate":"2005-08-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000230431000008","VABBcode":null,"OpenAcc":1,"DOI":"10.1007/s10126-004-0051-y"},"refs":null,"anarec":{"AnaID":75918,"PubliDate":2005,"Pagination":"207-214","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"3","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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