{"refrec":{"BRefID":75919,"RR":"<b>Kim, C.-J.; Kim, C.-H.; Sako, Y.</b> (2005). Development of molecular identification method for genus <i>Alexandrium</i> (Dinophyceae) using whole-cell FISH. <i>Mar. Biotechnol. 7(3)</i>: 215-222. <a href=\"https://dx.doi.org/10.1007/s10126-004-0424-2\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0424-2</a>","BEntID":71588,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(3)</i>: 215-222. <a href=\"https://dx.doi.org/10.1007/s10126-004-0424-2\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-0424-2</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Kim, C.-J.; Kim, C.-H.; Sako, Y.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Kim, C.-J.; Kim, C.-H.; Sako, Y.","Englishabstract":"We have developed a method to identify species in the genus <i>Alexandrium</i> using whole-cell fluorescent in situ hybridization with FITC-labeled oligonucleotide probes that target large subunit ribosomal rRNA molecules. The probes were designed based on the sequence of the rDNA D1-D2 region of <i>Alexandrium</i> species. DNA probes specific for toxic <i>A. tamarense</i> and <i>A. catenella</i> and nontoxic <i>A. affine</i>, <i>A. fraterculus</i>, <i>A. insuetum</i>, and <i>A. pseudogonyaulax</i>, respectively, were applied to vegetative cells of all above <i>Alexandrium</i> species to test the sensitivity of the probes. Each DNA probe hybridized specifically with vegetative cells of the corresponding <i>Alexandrium</i> species and showed no cross-reactivity to noncorresponding <i>Alexandrium</i> species. In addition, no cross-reactivity of the probes was observed in experiments using concentrated natural seawater samples. 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