Document of bibliographic reference 76183

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

In vitro estrogenicity of polybrominated flame retardants

Abstract

Estrogenicity of five brominated flame retardants (BFRs), namely BDE-47, BDE-99, BDE-205, PBB-153 and technical Firemaster BP-6™, were assessed by in vitro assays developed to detect chemicals with estrogenic properties. Recombinant yeast cells containing a human estrogen receptor gene failed to give any response to the chemicals tested. However, the positive control compound, estradiol-17β, showed that the yeast cell assays had worked properly. The freshly separated fish hepatocyte assay based on the synthesis and secretion of vitellogenin from the isolated liver cells produced a clear dose–response curve in the presence of all tested flame retardants except Firemaster BP-6™. The toxicity of the BFRs was detected by determining the cell ethoxyresorufin-O-deethylase activity (EROD). The BFRs tested induced hepatic EROD activity at low test concentrations, but started to inhibit activity at higher concentrations. The decreased detoxification capacity of the hepatocytes resulted in a decrease in the vitellogenin production of the cells. The capability of in vitro assays to detect estrogenic properties of chemicals seems to vary. Thus, further work is needed to understand the mechanisms responsible for these reactions.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000231522000008

Bibliographic citation

Nakari, T.; Pessala, P. (2005). In vitro estrogenicity of polybrominated flame retardants. Aquat. Toxicol. 74(3): 272-279. http://dx.doi.org/10.1016/j.aquatox.2005.06.004

location created

Hakuninmaantie 6

Topic

Marine

Is peer reviewed

true