{"refrec":{"BRefID":77346,"RR":"<b>de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E.</b> (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (<i>Phoca vitulina</i>). <i>Vet. Immunol. Immunopathol. 37(3-4)</i>: 217-230. <a href=\"https://dx.doi.org/10.1016/0165-2427(93)90195-A\" target=\"_blank\">https://dx.doi.org/10.1016/0165-2427(93)90195-A</a>","BEntID":72950,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Vet. Immunol. Immunopathol. 37(3-4)</i>: 217-230. <a href=\"https://dx.doi.org/10.1016/0165-2427(93)90195-A\" target=\"_blank\">https://dx.doi.org/10.1016/0165-2427(93)90195-A</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E.","OrigTitleTranslFlag":0,"Authorstringtrunc":"de Swart, R.L. <i>et al.</i>","Englishabstract":"In vitro assays were developed for studies concerning the functioning of the immune system of the harbour seal (<i>Phoca vitulina</i>). Proliferative responses of peripheral blood mononuclear cells (PBMC) were measured after stimulation with different concentrations of the mitogens concanavalin A (Con A), pokeweed mitogen (PWM), phytohaemagglutinin (PHA) or lipopolysaccharide from Salmonella typhimurium (LPS). Con A and PWM induced strong proliferative responses, which PHA and LPS induced comparatively low proliferative responses. Antigen-specific immune responses were measured after immunization of seals with an inactivated rabies vaccine and/or with tetanus toxoid. Antigen-specific proliferation of PBMC and the presence of antigen-specific antibody forming cells were demonstrated for both antigen s in the PBMC of immunized animals. The responses measured in vitro correlated well with the development of specific serum antibody titers to these antigens.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (<i>Phoca vitulina</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-22 01:31:33.008388","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1993,"MonPub":null,"DateUpdate":"2019-08-27","DateCreate":"2005-10-24","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:A1993LU59600003","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/0165-2427(93)90195-A"},"refs":null,"anarec":{"AnaID":77346,"PubliDate":1993,"Pagination":"217-230","XtraPublOfAnaID":null,"ISBN":null,"Volume":"37","Issue":"3-4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":44062,"SerRR":"Veterinary Immunology and Immunopathology. Elsevier: Tokyo; Oxford; Lausanne; New York; Shannon; Amsterdam.  ISSN 0165-2427; e-ISSN 1873-2534","StandardTitleSer":"Veterinary Immunology and Immunopathology","ISSN":"0165-2427","AbbrevSer":"Vet. Immunol. Immunopathol.","StandardTitleMon":null,"StartPage":217,"Pages":14,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":[{"YBRefID":77347,"RefStrFull":"<b>de Swart, R.L.; Kluten, R.M.G.; Huizing, C.J.; Vedder, L.J.; Reijnders, P.J.H.; Visser, I.K.G.; Uytdehaag, F.G.C.M.; Osterhaus, A.D.M.E.</b> (1993). Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (<i>Phoca vitulina</i>), <b><i>in</i></b>: de Swart, R.L. (1995). <i>Impaired immunity in seals exposed to bioaccumulated environmental contaminants = Immuunsuppressie in zeehonden blootgesteld aan in de voedselketen geaccumuleerde milieuvervuilende stoffen.</i> pp. 23-34","RelID":4,"Relation":"Related to","StandardTitle":"Mitogen and antigen induced B and T cell responses of peripheral blood mononuclear cells from the harbour seal (<i>Phoca vitulina</i>)"}],"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"de 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