{"refrec":{"BRefID":77949,"RR":"<b>Vaccaro, E.; Meucci, V.; Intorre, L.; Soldani, G.; Di Bello, D.; Longo, V.; Gervasi, P.G.; Pretti, C.</b> (2005). Effects of 17ß-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (<i>Dicentrarchus labrax</i>). <i>Aquat. Toxicol. 75(4)</i>: 293-305. <a href=\"http://dx.doi.org/10.1016/j.aquatox.2005.08.009\" target=\"_blank\">http://dx.doi.org/10.1016/j.aquatox.2005.08.009</a>","BEntID":73488,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Aquat. Toxicol. 75(4)</i>: 293-305. <a href=\"https://dx.doi.org/10.1016/j.aquatox.2005.08.009\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquatox.2005.08.009</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Vaccaro, E.; Meucci, V.; Intorre, L.; Soldani, G.; Di Bello, D.; Longo, V.; Gervasi, P.G.; Pretti, C.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Vaccaro, E. <i>et al.</i>","Englishabstract":"The endocrine system of wildlife is exposed to a wide variety of natural and man-made chemicals which may lead to damage to the reproductive system and other adverse effects, including alteration of drug-metabolizing enzymes. In the present study, the effects of in vivo exposure to a natural (17&#x3b2;-estradiol: E2) or a xenoestrogen (4-nonylphenol: NP) estrogen or an anti-estrogen (3,3&#x2032;,4,4&#x2032;,5-pentachlorobiphenyl: PCB 126) upon vitellogenin (VTG) synthesis and hepatic phase 1 and 2 enzymes have been investigated in adult male sea bass. By means of ELISA analysis with the use of polyclonal antibodies prepared against VTG purified from E2-treated sea bass, we assessed the time course and sensitivity of VTG induction in the plasma of sea bass treated with E2 at 0.1, 0.5, 2.5 and 5.0&nbsp;mg/kg doses or NP at 5.0 or 50&nbsp;mg/kg doses, respectively. Sea bass sensitivity to this induction was found to be similar to that of other fish species, but with a delay in maximal response. E2 treatment also caused a selective time- and dose-dependent inhibition of hepatic CYP1A-linked EROD and phase 2 glutathione <i>S</i>-transferase (GST) activities, without affecting the activity of CYP3A-linked 6&#x3b2;-testosterone hydroxylase, (&#x3c9;)- and (&#x3c9;-1)-lauric acid hydroxylases or phase 2 DT-diaphorase. A similar selective inhibition on CYP1A was also observed in fish treated with 50&nbsp;mg/kg NP. The results regarding CYP1A and CYP3A were also confirmed by Western blot analysis. When the sea bass were treated with either 10 or 100&nbsp;&#x3bc;g/kg PCB 126, an AhR ligand not yet tested in vivo in fish to assess its anti-estrogenicity, a modest and selective induction of EROD and DT-diaphorase activities was observed. Interestingly, both these activities were recovered to their control levels in sea bass co-treated with 0.5&nbsp;mg/kg E2 and 10 or 100&nbsp;&#x3bc;g/kg PCB 126, probably through a cross-talk mechanism between the estrogen receptor and AhR or other transcription factors that regulate the expression of these enzymes. Furthermore, it was demonstrated that PCB 126 possesses a potent anti-estrogenic activity in the sea bass in vivo as it inhibited the E2-induced VTG synthesis with an IC50 of 28&nbsp;&#x3bc;g/kg. The results of this study suggest that the exposure of fish to xenoestrogens or anti-estrogens may alter, in addition to various physiological processes, the expression of specific CYPs and phase 2 enzymes, thereby reducing the capability of their detoxication system.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Effects of 17ß-estradiol, 4-nonylphenol and PCB 126 on the estrogenic activity and phase 1 and 2 biotransformation enzymes in male sea bass (<i>Dicentrarchus labrax</i>)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"sea bass; Dicentrarchus labrax; vitellogenin; 17 beta-estradiol;4-nonylphenol; PCB 126; CYP1A; CYP3A; glutathione S-transferase;DT-diaphorase","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2018-05-17","DateCreate":"2005-11-28","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000233491200001","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2005.08.009"},"refs":null,"anarec":{"AnaID":77949,"PubliDate":2005,"Pagination":"293-305","XtraPublOfAnaID":null,"ISBN":null,"Volume":"75","Issue":"4","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam.  ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. 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