{"refrec":{"BRefID":78009,"RR":"<b>Pérez, F.; Ortiz, J.; Zhinaula, M.; Gonzabay, C.; Calderón, J.; Volckaert, F.A.M.J.</b> (2005). Development of EST-SSR markers by data mining in three species of shrimp: <i>Litopenaeus vannamei</i>, <i>Litopenaeus stylirostris</i>, and <i>Trachypenaeus birdy</i>. <i>Mar. Biotechnol. 7(5)</i>: 554-569. <a href=\"http://dx.doi.org/10.1007/s10126-004-5099-1\" target=\"_blank\">dx.doi.org/10.1007/s10126-004-5099-1</a>","BEntID":73548,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(5)</i>: 554-569. <a href=\"https://dx.doi.org/10.1007/s10126-004-5099-1\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-5099-1</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Pérez, F.; Ortiz, J.; Zhinaula, M.; Gonzabay, C.; Calderón, J.; Volckaert, F.A.M.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Pérez, F. <i>et al.</i>","Englishabstract":"We report on the data mining of publicly available <i>Litopenaeus vannamei</i> expressed sequence tags (ESTs) to generate simple sequence repeat (SSRs) markers and on their transferability between related Penaeid shrimp species. Repeat motifs were found in 3.8% of the evaluated ESTs at a frequency of one repeat every 7.8 kb of sequence data. A total of 206 primer pairs were designed, and 112 loci were amplified with the highest success in <i>L. vannamei</i>. A high percentage (69%) of EST-SSRs were transferable within the genus <i>Litopenaeus</i>. More than half of the amplified products were polymorphic in a small testing panel of <i>L. vannamei</i>. Evaluation of those primers in a larger testing panel showed that 72% of the markers fit Hardy-Weinberg equilibrium, which shows their utility for population genetic analysis. Additionally, a set of 26 of the EST-SSRs were evaluated for Mendelian segregation. A high percentage of monomorphic markers (46%) proved to be polymorphic by singles-stranded conformational polymorphism analysis. Because of the high number of ESTs available in public databases, a data mining approach similar to the one outlined here might yield high numbers of SSR markers in many animal taxa.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Development of EST-SSR markers by data mining in three species of shrimp: <i>Litopenaeus vannamei</i>, <i>Litopenaeus stylirostris</i>, and <i>Trachypenaeus birdy</i>","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"data mining; EST-SSR; linkage mapping; Litopenaeus; population genetics; type I markers","OtherDescriptors":null,"Notes":null,"AnaPub":2005,"MonPub":null,"DateUpdate":"2012-04-03","DateCreate":"2005-11-29","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000232866100015","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s10126-004-5099-1"},"refs":null,"anarec":{"AnaID":78009,"PubliDate":2005,"Pagination":"554-569","XtraPublOfAnaID":null,"ISBN":null,"Volume":"7","Issue":"5","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45426,"SerRR":"Marine Biotechnology. 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