{"refrec":{"BRefID":80449,"RR":"<b>Li, Q.; Kijima, A.</b> (2005). Segregation of microsatellite alleles in gynogenetic diploid Pacific abalone (<i>Haliotis discus hannai</i>). <i>Mar. Biotechnol. 7(6)</i>: 669-676. <a href=\"http://dx.doi.org/10.1007/s10126-004-5119-1\" target=\"_blank\">http://dx.doi.org/10.1007/s10126-004-5119-1</a>","BEntID":75951,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>Mar. Biotechnol. 7(6)</i>: 669-676. <a href=\"https://dx.doi.org/10.1007/s10126-004-5119-1\" target=\"_blank\">https://dx.doi.org/10.1007/s10126-004-5119-1</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Li, Q.; Kijima, A.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Li, Q.; Kijima, A.","Englishabstract":"Inheritance of 9 microsatellite loci was examined in 3 families of gynogenetic Pacific abalone <i>Haliotis discus hannai</i> produced by fertilizing eggs with UV-irradiated sperm followed by inhibition of the second meiotic division. The proportion of heterozygous progeny was used to estimate marker-centromere (M-C) distances. All loci conformed to Mendelian segregation in the control crosses when null alleles were accounted for. The absence of paternal alleles confirmed the gynogenetic origin of the offspring and indicated 100% success for 3 families. Estimated recombinant frequencies ranged from 0.10 to 0.60, which is lower than those observed in other gynogenetic diploid animals. The mean recombination frequency was 0.22, corresponding to a fixation index of 0.78 in one generation. This is 3.12 times the increase in homozygosity expected after one generation of sib mating (0.25), suggesting meiotic gynogenesis may be an effective means of rapid inbreeding in the abalone. M-C map distances for the 9 loci varied between 5 and 30 cM under the assumption of complete interference. 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