{"refrec":{"BRefID":8336,"RR":"<b>Inoue, I.</b> (1995). Resting and active K<sup>+</sup> channels in the squid axon membrane, <b><i>in</i></b>: Abbott, N.J. <i>et al.</i> <i>Cephalopod neurobiology: neuroscience studies in squid, octopus and cuttlefish.</i> pp. 73-83","BEntID":8336,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":0,"RefStringPartII":", <b><i>in</i></b>: Abbott, N.J. <i>et al.</i> <i>Cephalopod neurobiology: neuroscience studies in squid, octopus and cuttlefish.</i> pp. 73-83","DocTypID":17,"DocType":"Book chapters","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Inoue, I.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Inoue, I.","Englishabstract":"Potassium currents (I<sub>K</sub>) were recorded from giant axons of squid, <i>Sepioteuthis lessoniana</i> under voltage clamp. The K<sup>+</sup> accumulation in a space between outer membrane surface and surrounding Schwann cells layer, the so-called Frankenhauser-Hodgkin space (F-H space) due to K<sup>+</sup> efflux was completely removed by immersing the nerve fibre in a high osmolarity solution. Under such conditions I<sub>K</sub> displays the real time course of potassium conductance (g<sub>K</sub>) of the membrane. Even though the K<sup>+</sup> accumulation had been removed completely, I<sub>K</sub> still decayed after reaching a peak. This decay is due to g<sub>K</sub> inactivation, and is well fitted by a dual exponential function, with time constants of approximately 10 ms and of the order of 100 ms. Cytochalasin B applied intracellularly was found to block only one component of g<sub>K</sub> (with the slow inactivation) in a concentration-dependent manner. At 50 µM cytochalasin B, g<sub>K</sub> lost the slow inactivation component, thus the decaying g<sub>K</sub> could be fitted by a single exponential function with time constant of approximately 10 ms. The cytochalasin B-sensitive component had quite different characteristics from the delayed rectifier g<sub>K</sub>: 1) This component was activated along a single exponential time-course with a time constant of 2 ms at 16.5°C following depolarization and then it decayed slowly toward a steady state. 2) Prepulses of -140 to -60 mV did not alter the time course of the cytochalasin B sensitive g<sub>K</sub>. Because of the slow activation of the drug-sensitive g<sub>K</sub>, cytochalasin B did not affect the time course of the action potential. On the other hand, cytochalasin B greatly decreased the steady-state K<sup>+</sup> conductance. These results suggest that g<sub>K</sub> of the squid axon membrane consists of at least two components, i.e. a delayed rectifier K<sup>+</sup> channel and a steady-state K<sup>+</sup> channel. The cytochalasin B resistant g<sub>K</sub> may represent the real time- and voltage-dependent characteristics of the delayed rectifier.","AbstractOtherLang":null,"BibLvlCode":"AM","StandardTitle":"Resting and active K<sup>+</sup> channels in the squid axon membrane","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":1995,"MonPub":null,"DateUpdate":"2019-10-29","DateCreate":"2001-05-07","SecASFANote":null,"ConfID":null,"PeerRev":0,"VlizCoreFlag":1,"WoScode":null,"VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":8336,"PubliDate":1995,"Pagination":"73-83","XtraPublOfAnaID":null,"ISBN":"0-19-854790-0","Volume":null,"Issue":null,"BRefMon":8320,"BRefMonRR":"<b>Abbott, N.J.; Williamson, R.; Maddock, L. (Ed.)</b> (1995). Cephalopod neurobiology: Neuroscience studies in squid, octopus and cuttlefish. Oxford University Press: London. ISBN 0-19-854790-0. 542 pp. <a href=\"https://dx.doi.org/10.1093/acprof:oso/9780198547907.001.0001\" target=\"_blank\">https://dx.doi.org/10.1093/acprof:oso/9780198547907.001.0001</a>","BRefXtra":null,"BRefXtraRR":null,"SerBRefID":null,"SerRR":null,"StandardTitleSer":null,"ISSN":null,"AbbrevSer":null,"StandardTitleMon":"Cephalopod neurobiology: Neuroscience studies in squid, octopus and cuttlefish","StartPage":73,"Pages":-19,"ToPubliDate":null,"BRefBibLvlCode":"M","SerNotes":null,"AutString":"Abbott, N.J.; Williamson, R.; Maddock, L. 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