{"refrec":{"BRefID":8482,"RR":"<b>Zietara, M.S.; Arndt, A.; Geets, A.; Hellemans, B.; Volckaert, F.A.M.J.</b> (2000). The nuclear rDNA region of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i> (Monogenea: Gyrodactylidae). <i>J. Parasitol. 86(6)</i>: 1368-1373. <a href=\"https://dx.doi.org/10.2307/3285031\" target=\"_blank\">https://dx.doi.org/10.2307/3285031</a>","BEntID":8482,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":null,"RefStringPartII":". <i>J. Parasitol. 86(6)</i>: 1368-1373. <a href=\"https://dx.doi.org/10.2307/3285031\" target=\"_blank\">https://dx.doi.org/10.2307/3285031</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Zietara, M.S.; Arndt, A.; Geets, A.; Hellemans, B.; Volckaert, F.A.M.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Zietara, M.S. <i>et al.</i>","Englishabstract":"The primary structure of the ribosomal DNA internal transcribed spacers (ITS-1 and ITS-2) and 5.8S rRNA gene were used to characterize and identify 2 monogenean species of <i>Gyrodactylus</i> living externally on the threespine stickleback (<i>Gasterosteus aculeatus</i>). The ITS region was amplified by PCR from freshwater, brackish, and marine isolates of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i>, and the ends of the coding regions were identified by comparative alignment. No intraspecific and very low interspecific variation were observed in the 5.8S rRNA gene; high inter- and low intraspecific variation were revealed in the ITS-1 and ITS-2 regions. The morphological species identification was in all cases confirmed by the molecular identification. Intraspecifically, samples from 2 locations in the North Sea could be differentiated, but the Baltic sample resembled North Sea genotypes. Our approach offers perspectives for a multimetric genetical, morphometrical, and ecological taxonomy of the genus <i>Gyrodactylus</i>.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"The nuclear rDNA region of <i>Gyrodactylus arcuatus</i> and <i>G. branchicus</i> (Monogenea: Gyrodactylidae)","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-18 01:31:09.577729","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":"Spacer region; Nucleotide sequence; Taxonomy; rRNA 5.8S; DNA; Molecular structure; <i>Gasterosteus aculeatus; Gyrodactylus branchicus; Gyrodactylus arcuatus; Gyroodactylus branchicus</i>","Notes":null,"AnaPub":2000,"MonPub":null,"DateUpdate":"2018-03-12","DateCreate":"2001-05-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000166184200035","VABBcode":null,"OpenAcc":0,"DOI":"10.2307/3285031"},"refs":null,"anarec":{"AnaID":8482,"PubliDate":2000,"Pagination":"1368-1373","XtraPublOfAnaID":null,"ISBN":null,"Volume":"86","Issue":"6","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43248,"SerRR":"The Journal of Parasitology. 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