{"refrec":{"BRefID":9527,"RR":"<b>Thyssen, A.; Van Eygen, S.; Hauben, L.; Goris, J.; Swings, J.; Ollevier, F.P.</b> (2000). Application of AFLP for taxonomic and epidemiological studies of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. <i>Int. J. Syst. Bacteriol. 50</i>: 1013-1019","BEntID":9527,"PublicFlag":1,"CheckedFlag":0,"wosflag":0,"vabbflag":null,"RefStringPartII":". <i>Int. J. Syst. Bacteriol. 50</i>: 1013-1019","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Thyssen, A.; Van Eygen, S.; Hauben, L.; Goris, J.; Swings, J.; Ollevier, F.P.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Thyssen, A. <i>et al.</i>","Englishabstract":"A collection of 106 <i>Photobacterium damselae</i> subsp. <i>piscicida</i> strains and 19 <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases <i>Hind</i>III and <i>Taq</i>I. Using numerical analysis, six clusters were recognized. The largest cluster (n=106) contained the majority of the strains tested and consisted exclusively of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. The <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains fell outside this cluster. DNA-DNA hybridization experiments showed 77% DNA binding between the two subspecies, indicating a close genetic relationship. This clearly demonstrates the applicability of AFLP in studying the taxonomic position of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. 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