{"refrec":{"BRefID":98638,"RR":"Gonzalez, H.O.; Roling, J.A.; Baldwin, W.S.; Bain, L.J. (2006). Physiological changes and differential gene expression in mummichogs (Fundulus heteroclitus) exposed to arsenic. Aquat. Toxicol. 77(1): 43-52. https://dx.doi.org/10.1016/j.aquatox.2005.10.014","BEntID":93949,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". Aquat. Toxicol. 77(1): 43-52. https://dx.doi.org/10.1016/j.aquatox.2005.10.014","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":1,"BrackishFlag":1,"TerrestrialFlag":0,"Authorstring":"Gonzalez, H.O.; Roling, J.A.; Baldwin, W.S.; Bain, L.J.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Gonzalez, H.O. et al.","Englishabstract":"Arsenic has been detected as a contaminant in water bodies around the world. Although a number of studies have shown toxicity to adult fish, little is known about its effects on the offspring. However, human epidemiological studies have shown that arsenic increases the number of stillbirths and prematurely born infants. We examined changes in the morphology and gene expression in juvenile mummichogs (Fundulus heteroclitus) whose parents were exposed to 230 ppb arsenic for 10 days immediately prior to spawning. The hatchlings of exposed fish had a 2.8-fold increased incidence of curved or stunted tails. Total RNA from 6-week-old hatchlings, reared in clean water, was used to construct a cDNA subtractive hybridization library. Using this library, we found 13 genes whose expression was altered in the hatchlings as a result of arsenic exposure. We confirmed differential expression by real-time PCR and found significant up-regulation of myosin light chain 2 (4.2-fold), type II keratin (1.5-fold), tropomyosin (3.1-fold) and parvalbumin (3.5-fold) in the hatchlings whose parents were exposed to arsenic. These genes are important during embryogenesis and their differential expression may be linked to the morphological changes observed in the hatchlings.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Physiological changes and differential gene expression in mummichogs (Fundulus heteroclitus) exposed to arsenic","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-05-28 01:30:56.469002","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"arsenic; Fundulus heteroclitus; subtractive hybridization; myosin lightchain 2; type II keratin; tropomyosin; parvalbumin","OtherDescriptors":null,"Notes":null,"AnaPub":2006,"MonPub":null,"DateUpdate":"2020-10-19","DateCreate":"2006-05-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000236831100005","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquatox.2005.10.014"},"refs":null,"anarec":{"AnaID":98638,"PubliDate":2006,"Pagination":"43-52","XtraPublOfAnaID":null,"ISBN":null,"Volume":"77","Issue":"1","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42203,"SerRR":"Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514","StandardTitleSer":"Aquatic Toxicology","ISSN":"0166-445X","AbbrevSer":"Aquat. Toxicol.","StandardTitleMon":null,"StartPage":43,"Pages":10,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Department of Biological Sciences","Email":"ibain@utep.edu","Line2":null,"EnvName":"USA"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Gonzalez","Firstname":"Horacio","Initials":"H.O.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":93949,"AutID":66106,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Roling","Firstname":"Jonathan","Initials":"J.A.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":93949,"AutID":43712,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Baldwin","Firstname":"William","Initials":"W.S.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":93949,"AutID":43715,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Bain","Firstname":"Lisa","Initials":"L.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":93949,"AutID":43475,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":1,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":352804,"LibID":36,"BRefID":98638,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"352804","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"
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