{"refrec":{"BRefID":99268,"RR":"<b>Jarman, S.N.; Gales, N.J.; Tierney, M.; Gill, P.C.; Elliott, N.G.</b> (2002). A DNA-based method for identification of krill species and its application to analysing the diet of marine vertebrate predators. <i>Mol. Ecol. 11(12)</i>: 2679-2690","BEntID":94530,"PublicFlag":1,"CheckedFlag":0,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Mol. Ecol. 11(12)</i>: 2679-2690","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Jarman, S.N.; Gales, N.J.; Tierney, M.; Gill, P.C.; Elliott, N.G.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Jarman, S.N. <i>et al.</i>","Englishabstract":"Accurate identification of species that are consumed by vertebrate predators is necessary for understanding marine food webs. Morphological methods for identifying prey components after consumption often fail to make accurate identifications of invertebrates because prey morphology becomes damaged during capture, ingestion and digestion. Another disadvantage of morphological methods for prey identification is that they often involve sampling procedures that are disruptive for the predator, such as stomach flushing or lethal collection. We have developed a DNA-based method for identifying species of krill (Crustacea: Malacostraca), an enormously abundant group of invertebrates that are directly consumed by many groups of marine vertebrates. The DNA-based approach allows identification of krill species present in samples of vertebrate stomach contents, vomit, and, more importantly, faeces. Utilizing samples of faeces from vertebrate predators minimizes the impact of dietary studies on the subject animals. We demonstrate our method first on samples of Adelie penguin (<i>Pygoscelis adeliae</i>) stomach contents, where DNA-based species identification can be confirmed by prey morphology. We then apply the method to faeces of Adelie penguins and to faeces of the endangered pygmy blue whale (<i>Balaenoptera musculus brevicauda</i>). In each of these cases, krill species consumed by the predators could be identified from their DNA present in faeces or stomach contents.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"A DNA-based method for identification of krill species and its application to analysing the diet of marine vertebrate predators","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:01.897972","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":null,"OtherDescriptors":null,"Notes":null,"AnaPub":2002,"MonPub":null,"DateUpdate":"2006-05-30","DateCreate":"2006-05-30","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000179492800020","VABBcode":null,"OpenAcc":0},"refs":null,"anarec":{"AnaID":99268,"PubliDate":2002,"Pagination":"2679-2690","XtraPublOfAnaID":null,"ISBN":null,"Volume":"11","Issue":"12","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":45425,"SerRR":"Molecular Ecology. Blackwell: Oxford.  ISSN 0962-1083; e-ISSN 1365-294X","StandardTitleSer":"Molecular Ecology","ISSN":"0962-1083","AbbrevSer":"Mol. Ecol.","StandardTitleMon":null,"StartPage":2679,"Pages":12,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":{"Line1":"Channel Highway","Email":null,"Line2":null,"EnvName":"Australia"},"othpubs":null,"ownerships":null,"authors":[{"AutName":"Jarman","Firstname":"Simon","Initials":"S.N.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":94530,"AutID":10032,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gales","Firstname":"Nicholas","Initials":"N.J.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":94530,"AutID":53230,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Tierney","Firstname":null,"Initials":"M.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":94530,"AutID":3590,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Gill","Firstname":null,"Initials":"P.C.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":94530,"AutID":66484,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null},{"AutName":"Elliott","Firstname":null,"Initials":"N.G.","Affiliation":null,"Discriminator":null,"CorporateFlag":0,"BEntID":94530,"AutID":66485,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":null,"ORCID":null,"PersID":null,"InsID":null}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":null,"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Blackwell","City":"Oxford"}],"MONpubls":null,"pictures":[],"thestermsPath":[{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTermPath":"Acids > Organic compounds > Organic acids > Nucleic acids > DNA"},{"ThesaurusTerm":"Genetic analysis","ThestID":110555,"Acronym":"CAB","ThesTermPath":"Analysis > Genetic analysis"},{"ThesaurusTerm":"Predators","ThestID":6508,"Acronym":"ASFA","ThesTermPath":"Aquatic organisms > Heterotrophic organisms > Predators"},{"ThesaurusTerm":"Marine birds","ThestID":4992,"Acronym":"ASFA","ThesTermPath":"Aquatic organisms > Marine organisms > Aquatic birds > Marine birds"},{"ThesaurusTerm":"Animal morphology","ThestID":396,"Acronym":"ASFA","ThesTermPath":"Biology > Organism morphology > Animal morphology"},{"ThesaurusTerm":"Diets","ThestID":2312,"Acronym":"ASFA","ThesTermPath":"Diets"},{"ThesaurusTerm":"Faecal pellets","ThestID":3022,"Acronym":"ASFA","ThesTermPath":"Excretory products > Faecal pellets"},{"ThesaurusTerm":"Marine crustaceans","ThestID":4995,"Acronym":"ASFA","ThesTermPath":"Fauna > Aquatic organisms > Aquatic animals > Shellfish > Marine organisms > Marine crustaceans"},{"ThesaurusTerm":"Feces","ThestID":123290,"Acronym":"CAB","ThesTermPath":"Feces"},{"ThesaurusTerm":"Feces","ThestID":59775,"Acronym":"CSA","ThesTermPath":"Feces"},{"ThesaurusTerm":"Food webs","ThestID":3409,"Acronym":"ASFA","ThesTermPath":"Food webs"},{"ThesaurusTerm":"Identification","ThestID":4228,"Acronym":"ASFA","ThesTermPath":"Identification"},{"ThesaurusTerm":"Predation","ThestID":6506,"Acronym":"ASFA","ThesTermPath":"Interspecific relationships > Predation"},{"ThesaurusTerm":"Methodology","ThestID":5176,"Acronym":"ASFA","ThesTermPath":"Methodology"},{"ThesaurusTerm":"Morphometry","ThestID":5351,"Acronym":"ASFA","ThesTermPath":"Morphometry"},{"ThesaurusTerm":"Stomach content","ThestID":8038,"Acronym":"ASFA","ThesTermPath":"Stomach content"}],"thestermsASFA":[{"ThesaurusTerm":"Animal morphology"},{"ThesaurusTerm":"Diets"},{"ThesaurusTerm":"DNA"},{"ThesaurusTerm":"Faecal pellets"},{"ThesaurusTerm":"Feces"},{"ThesaurusTerm":"Feces"},{"ThesaurusTerm":"Food webs"},{"ThesaurusTerm":"Genetic analysis"},{"ThesaurusTerm":"Identification"},{"ThesaurusTerm":"Marine birds"},{"ThesaurusTerm":"Marine crustaceans"},{"ThesaurusTerm":"Methodology"},{"ThesaurusTerm":"Morphometry"},{"ThesaurusTerm":"Predation"},{"ThesaurusTerm":"Predators"},{"ThesaurusTerm":"Stomach content"}],"taxtermsASFA":[{"TaxTerm":"Balaenoptera musculus"},{"TaxTerm":"Balaenoptera musculus brevicauda"},{"TaxTerm":"Euphausiacea"},{"TaxTerm":"Pygoscelis adeliae"}],"geotermsASFA":null,"collections":null,"conf":null,"proj":null,"Physdatasets":null,"spcols":null,"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":94530,"AbstractFlag":-1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":null,"thesterms":[{"ThesaurusTerm":"Animal morphology","ThestID":396,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Diets","ThestID":2312,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Faecal pellets","ThestID":3022,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Feces","ThestID":59775,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Feces","ThestID":123290,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Food webs","ThestID":3409,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Genetic analysis","ThestID":110555,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Identification","ThestID":4228,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine birds","ThestID":4992,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine crustaceans","ThestID":4995,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Methodology","ThestID":5176,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Morphometry","ThestID":5351,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Predation","ThestID":6506,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Predators","ThestID":6508,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Stomach content","ThestID":8038,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxterms":[{"TaxTerm":"Balaenoptera musculus","AphiaID":137090,"TaxtID":23558},{"TaxTerm":"Balaenoptera musculus brevicauda [pygmy blue whale]","AphiaID":242604,"TaxtID":35716},{"TaxTerm":"Euphausiacea [krill]","AphiaID":1128,"TaxtID":15617},{"TaxTerm":"Pygoscelis adeliae [Adélie penguin]","AphiaID":225757,"TaxtID":35715}],"geoterms":null,"othterms":[{"OtherTerm":"Dietary intake","OthtID":16059}],"asfacodes":null,"asfa2codes":null,"thestermsFRIS":[{"ThesaurusTerm":"Animal morphology","DutchTerm":"Dierlijke morfologie","ThestID":396,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Diets","DutchTerm":"Diëten","ThestID":2312,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"DNA","DutchTerm":"DNA","ThestID":2425,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Faecal pellets","DutchTerm":"Fecale pellets","ThestID":3022,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Feces","DutchTerm":null,"ThestID":59775,"Acronym":"CSA","ThesTypID":2,"ThesType":"CSA Technology Research Database Master Thesaurus"},{"ThesaurusTerm":"Feces","DutchTerm":null,"ThestID":123290,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Food webs","DutchTerm":"Voedselketen","ThestID":3409,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Genetic analysis","DutchTerm":null,"ThestID":110555,"Acronym":"CAB","ThesTypID":4,"ThesType":"CAB Thesaurus"},{"ThesaurusTerm":"Identification","DutchTerm":"Identificatie","ThestID":4228,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine birds","DutchTerm":"Zeevogels","ThestID":4992,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Marine crustaceans","DutchTerm":"Mariene crustaceeën","ThestID":4995,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Methodology","DutchTerm":"methodologie","ThestID":5176,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Morphometry","DutchTerm":null,"ThestID":5351,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Predation","DutchTerm":"Predatie","ThestID":6506,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Predators","DutchTerm":"Predatoren","ThestID":6508,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"},{"ThesaurusTerm":"Stomach content","DutchTerm":"Maaginhoud","ThestID":8038,"Acronym":"ASFA","ThesTypID":1,"ThesType":"ASFA Thesaurus List"}],"taxtermsFRIS":[{"TaxTerm":"Balaenoptera musculus","DutchTerm":"Blauwe vinvis","AphiaID":137090,"TaxtID":23558},{"TaxTerm":"Balaenoptera musculus brevicauda","DutchTerm":null,"AphiaID":242604,"TaxtID":35716},{"TaxTerm":"Euphausiacea","DutchTerm":"Krill","AphiaID":1128,"TaxtID":15617},{"TaxTerm":"Pygoscelis adeliae","DutchTerm":"Adélie-pinguïn","AphiaID":225757,"TaxtID":35715}],"geotermsFRIS":null,"othtermsFRIS":[{"OtherTerm":"Dietary intake","DutchTerm":null,"OthtID":16059}],"resmessage":"","complete":1,"sessions":{"newSesName":"Milh, Dominique, D.","newSesDate":{"date":"2006-05-30 10:21:17.147000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Milh, Dominique, D.","updSesDate":{"date":"2006-05-30 10:21:17.147000","timezone_type":3,"timezone":"Europe/Brussels"}}}