Project record of Development of DNA probes to determine the genetic sex in aquacultured fish species

Information

Type
Project
Name
Development of DNA probes to determine the genetic sex in aquacultured fish species
Name @nl
Ontwikkeling van DNA probes om het genetisch geslacht van gekweekte vissoorten te bepalen
Description @en
We will produce DNA probes that identify unambiguously the genetic sex of European eel. Both species' genetic and phenotypic sex do not necessarely match; moreover sex inversions occur commonly, such probes will facilitate selection of suitable fish for aquaculture. Genotypic and phenotypic sex do not necessarily coincide in fish; very often sex inversions or environmentally induced sex differentiation are observed. As a consequence many aquacultured stocks show an unbalanced distribution of the sexes. If the genotypic sex would be known, a strategy could be more easily developed to influence sex differentation and to develop monosex populations. Our strategy to identify the genotypic sex in fish includes two major goals: (1) the development of DNA probes of repetitive non-coding sequences associated with differentiated sex chromosomes and (2) the development of DNA probes of sequences related to the sex determining gene. The fish species Leporinus, Poecilia (guppy) and Oncorhynchus (rainbow trout) will be used as reference species (they carry well studied gonosomes), while Dicentrarchus (sea bass) and Anguilla (European eel) are the target species. Such information will facilitate aquaculture by providing effective means to monitor the mechanisms which cause the switching of phenotypic sex. It is expected that at the end of the project various probes will be available to the aquaculture community which are suitable for identifying the genotypic sex of sea bass, European eel and possibly other commercial species. We will also procude probes based on the sex gene proper which could be diagnostic in other fish species.
Description @nl
Wij ontwikkelen DNA sondes die eenduidig de genetische sex van de Europese paling bepalen. Het genetische en fenotypische geslacht van beide soorten komen niet noodzakelijk overeen, bovendien komen geslachtsomkeringen vaak voor. Dergelijke sondes zullen de selectie van vis voor aquacultuur vergemakkelijken.
Start date
1994-1
End date
1996-12

Institutes & people involved

Volckaert Filip
associated with: Katholieke Universiteit Leuven; Departement Biologie; Afdeling Dierenecologie en -systematiek; Diversiteit en Systematiek van Dieren
Ollevier Frans
associated with: Katholieke Universiteit Leuven; Departement Biologie; Afdeling Dierenecologie en -systematiek; Diversiteit en Systematiek van Dieren

Expertise

Aquaculture
defined term set: ASFA Thesaurus List
term code: 473
Fish
defined term set: ASFA Thesaurus List
term code: 3141
Sex
defined term set: ASFA Thesaurus List
term code: 7519
taxonomic terms
Anguilla anguilla
Dicentrarchus labrax [Bass]
Oncorhynchus mykiss (Walbaum, 1792)
Poecilia
geographic terms
Belgium

special collections

Marine Biodiversity and Ecosystem Functioning

record metadata

date created: 2003-11-26
date modified: 2007-04-02