Document of bibliographic reference 303981

BibliographicReference record

Type

Bibliographic resource

Type of document

Journal article

BibLvlCode

AS

Title

A polyphasic approach to the delimitation of diatom species: a case study for the genus Pinnularia (Bacillariophyta)

Abstract

Diatoms are one of the most abundant and arguably the most species‐rich group of protists. Diatom species delimitation has been often based exclusively on the recognition of morphological discontinuities without investigation of other lines of evidence. Even though DNA sequences and reproductive experiments have revealed several examples of (pseudo)cryptic diversity, our understanding of diatom species boundaries and diversity remains limited. The cosmopolitan pennate raphid diatom genus Pinnularia represents one of the most taxon‐rich diatom genera. In this study, we focused on the delimitation of species in one of the major clades of the genus, the Pinnularia subgibba group, based on 105 strains from a worldwide origin. We compared genetic distances between the sequences of seven molecular markers and selected the most variable pair, the mitochondrial cox1 and nuclear encoded LSU rDNA, to formulate a primary hypothesis on the species limits using three single‐locus automated species delimitation methods. We compared the DNA‐based primary hypotheses with morphology and with other available lines of evidence. The results indicate that our dataset comprised 15 species of the P. subgibba group. The vast majority of these taxa have an uncertain taxonomic identity, suggesting that several may be unknown to science and/or members of (pseudo)cryptic species complexes within the P. subgibba group.

WebOfScience code

https://www.webofscience.com/wos/woscc/full-record/WOS:000465097000010

Bibliographic citation

Kollár, J.; Pinseel, E.; Vanormelingen, P.; Poulícková, A.; Souffreau, C.; Dvorák, P.; Vyverman, W. (2019). A polyphasic approach to the delimitation of diatom species: a case study for the genus Pinnularia (Bacillariophyta). J. Phycol. 55(2): 365-379. https://dx.doi.org/10.1111/jpy.12825

Topic

Marine

Is peer reviewed

true