{"refrec":{"BRefID":327922,"RR":"<b>Rahmani, A.; Vercauteren, M.; Vranckx, K.; Boyen, F.; Bidault, A.; Pichereau, V.; Decostere, A.; Paillard, C.; Chiers, K.</b> (2021). MALDI-TOF MS as a promising tool to assess potential virulence of <i>Vibrio tapetis</i> isolates. <i>Aquaculture 530</i>: 735729. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2020.735729\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2020.735729</a>","BEntID":321399,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Aquaculture 530</i>: 735729. <a href=\"https://dx.doi.org/10.1016/j.aquaculture.2020.735729\" target=\"_blank\">https://dx.doi.org/10.1016/j.aquaculture.2020.735729</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Rahmani, A.; Vercauteren, M.; Vranckx, K.; Boyen, F.; Bidault, A.; Pichereau, V.; Decostere, A.; Paillard, C.; Chiers, K.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Rahmani, A. <i>et al.</i>","Englishabstract":"<em>Vibrio tapetis</em>, the etiological agent of Brown Ring Disease, mainly affects the Manila clam <em>Ruditapes philippinarum.</em> Although this bacterium is mainly known as a clam pathogen, it has been isolated from several fish species. The main aim of the present study was to further explore the variability of 27 <em>V. tapetis</em> isolates from bivalves and fish, considering three different aspects; <em>in vitro</em> virulence based on the loss of clam hemocyte adhesion properties, detection of the gene <em>virB4</em> encoding for an essential component of the Type IV Secretion System, and MALDI-TOF MS characterization based on whole cell extracts. Finally, these approaches were compared and evaluated for their ability to differentiate the potential pathogenicity of the 27 isolates against the Manila clams. Among the 11 <em>V. tapetis</em> isolates from the common dab isolated in 2018 in Belgium, only one (2BB) showed intermediate <em>in vitro</em> virulence against the Manila clam, and seven carried the <em>virB4</em> gene while none of the <em>V. tapetis</em> previously isolated from fish in 2003 showed the presence of this particular gene. Finally, the peak protein profiles generated with MALDI-TOF MS analysis from all 27 <em>V. tapetis</em> strains showed a clear clustering of clam pathogenic and nonpathogenic isolates suggesting that a new isolate of <em>V. tapetis</em> that would cluster within the clam pathogenic isolates could be potentially pathogenic to the Manila clam. Thereupon, MALDI-TOF MS typing allows rapid and cost-efficient identification of <em>V. tapetis</em> isolates and can be defined as a complementary method of the traditional qPCR that opens new perspectives to study the virulence of <em>V. tapetis</em> isolates but also to perform environmental monitoring in order to prevent outbreaks.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"MALDI-TOF MS as a promising tool to assess potential virulence of <i>Vibrio tapetis</i> isolates","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2024-12-10 01:33:17.368041","timezone_type":1,"timezone":"+01:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Vibrio tapetis; MALDI-TOF MS; virB4; Hemocyte cytotoxicity assay; Manila clam; Fish","OtherDescriptors":null,"Notes":null,"AnaPub":2021,"MonPub":null,"DateUpdate":"2021-06-10","DateCreate":"2020-08-12","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000582169700047","VABBcode":null,"OpenAcc":0,"DOI":"10.1016/j.aquaculture.2020.735729"},"refs":null,"anarec":{"AnaID":327922,"PubliDate":2021,"Pagination":"735729","XtraPublOfAnaID":null,"ISBN":null,"Volume":"530","Issue":null,"BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42190,"SerRR":"Aquaculture. 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