{"refrec":{"BRefID":336135,"RR":"<b>Ehsani, E.; Dumolin, C.; Arends, J.B.A.; Kerckhof, F.-M.; Hu, X.; Vandamme, P.; Boon, N.</b> (2019). Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria. <i>Appl. Microbiol. Biotechnol. 103(19)</i>: 8241-8253. <a href=\"https://dx.doi.org/10.1007/s00253-019-10082-z\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-019-10082-z</a>","BEntID":332757,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":1,"RefStringPartII":". <i>Appl. Microbiol. Biotechnol. 103(19)</i>: 8241-8253. <a href=\"https://dx.doi.org/10.1007/s00253-019-10082-z\" target=\"_blank\">https://dx.doi.org/10.1007/s00253-019-10082-z</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":0,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Ehsani, E.; Dumolin, C.; Arends, J.B.A.; Kerckhof, F.-M.; Hu, X.; Vandamme, P.; Boon, N.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Ehsani, E. <i>et al.</i>","Englishabstract":"While numerous reports exist on the axenic culturing of different hydrogen-oxidizing bacteria (HOB), knowledge about the enrichment of microbial communities growing on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources remains negligible. We want to elucidate if in such enrichments, most enriched populations are HOBs or heterotrophic organisms. In the present study, bacteria enriched from a soil sample and grown over 5 transfers using a continuous supply of hydrogen, oxygen, and carbon dioxide to obtain an enriched autotrophic hydrogen-oxidizing microbiome. The success of the enrichment was evaluated by monitoring ammonium consumption and biomass concentration for 120 days. The shift in the microbial composition of the original soil inoculum and all transfers was observed based on 16S rRNA amplicon sequencing. The hydrogen-oxidizing facultative chemolithoautotroph <i>Hydrogenophaga electricum</i> was isolated and found to be one of the abundant species in most transfers. Moreover, <i>Achromobacter</i> was isolated both under heterotrophic and autotrophic conditions, which was characterized as a hydrogen-oxidizing bacterium. The HOB enrichment condition constructed in this study provided an environment for HOB to develop and conquer in all transfers. In conclusion, we showed that enrichments on hydrogen, oxygen, and carbon dioxide as sole carbon and energy sources contain a diverse mixture of HOB and heterotrophs that resulted in a collection of culturable isolates. These isolates can be useful for further investigation for industrial applications.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Enriched hydrogen-oxidizing microbiomes show a high diversity of co-existing hydrogen-oxidizing bacteria","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-06-10 01:32:34.728526","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"Hydrogen-oxidizing bacteria; HOB enrichment; HOB isolation; Microbialcomposition; Heterotrophs","OtherDescriptors":null,"Notes":null,"AnaPub":2019,"MonPub":null,"DateUpdate":"2022-12-07","DateCreate":"2021-04-08","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:000485936100033","VABBcode":null,"OpenAcc":0,"DOI":"10.1007/s00253-019-10082-z"},"refs":null,"anarec":{"AnaID":336135,"PubliDate":2019,"Pagination":"8241-8253","XtraPublOfAnaID":null,"ISBN":null,"Volume":"103","Issue":"19","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":42367,"SerRR":"Applied Microbiology and Biotechnology. Springer: Heidelberg; Berlin.  ISSN 0175-7598; e-ISSN 1432-0614","StandardTitleSer":"Applied Microbiology and Biotechnology","ISSN":"0175-7598","AbbrevSer":"Appl. Microbiol. Biotechnol.","StandardTitleMon":null,"StartPage":8241,"Pages":13,"ToPubliDate":null,"BRefBibLvlCode":"S","SerNotes":null},"monrec":null,"serrec":null,"relations":null,"relationsRev":null,"addrec":null,"othpubs":null,"ownerships":null,"authors":[{"AutName":"Ehsani","Firstname":"Elham","Initials":"E.","Affiliation":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441552,"OrderNr":1,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology","ORCID":null,"PersID":39903,"InsID":6702},{"AutName":"Dumolin","Firstname":"Charles","Initials":"C.","Affiliation":"Laboratory for Microbiology (LM-UGent), Faculty of Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441553,"OrderNr":2,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":"0000-0002-2691-0964","PersID":34606,"InsID":1605},{"AutName":"Arends","Firstname":"Jan","Initials":"J.B.A.","Affiliation":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441554,"OrderNr":3,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology","ORCID":"0000-0001-7035-2587","PersID":27665,"InsID":6702},{"AutName":"Kerckhof","Firstname":"Frederiek-Maarten","Initials":"F.-M.","Affiliation":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441555,"OrderNr":4,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology","ORCID":"0000-0002-4472-6810","PersID":33513,"InsID":6702},{"AutName":"Hu","Firstname":"Xiaona","Initials":"X.","Affiliation":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441556,"OrderNr":5,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology","ORCID":"0000-0002-4323-6932","PersID":39909,"InsID":6702},{"AutName":"Vandamme","Firstname":"Peter","Initials":"P.","Affiliation":"Laboratory for Microbiology (LM-UGent), Faculty of Sciences, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441557,"OrderNr":6,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":null,"InsFSN":"Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biochemie en Microbiologie; Laboratorium voor Microbiologie","ORCID":"0000-0002-5581-7937","PersID":10795,"InsID":1605},{"AutName":"Boon","Firstname":"Nico","Initials":"N.","Affiliation":"Center for Microbial Ecology and Technology (CMET), Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University","Discriminator":null,"CorporateFlag":0,"BEntID":332757,"AutID":441533,"OrderNr":7,"DegrID":null,"EditorFlag":0,"CorrespFlag":0,"IllustratorFlag":0,"ReviserFlag":0,"TranslatorFlag":0,"InsAcronym":"CMET","InsFSN":"Ghent University; Faculty of Bioscience Engineering; Department of Biotechnology; Center for Microbial Ecology and Technology","ORCID":"0000-0002-7734-3103","PersID":15308,"InsID":6702}],"mapdetails":null,"datasets":null,"monographs":null,"monparts":null,"serparts":null,"BEntOpen":null,"BEntPrivate":null,"availability":[{"BInstID":360069,"LibID":36,"BRefID":336135,"EmbargoDate":null,"FullEmbargoDate":null,"PhysMedID":16,"hasOCRd":null,"ShelfLocCode":"360069","RFID":null,"PaidValue":null,"Medium":"Server","Description":"Interne VLIZ documenten","Acronym":"VLIZ","Library":"Vlaams Instituut voor de Zee","DutchTerm":"Non-open access","URL":null,"ClassifID":228,"Classification":"Non-open access","ReqLink":1,"ClassifTypID":3,"URLLocation":"https://www.vliz.be/imisdocs/publications/","SubDir":1,"InternalReq":1,"LoggedInReq":1,"Disclaimer":"Disclaimer_VLIZ_Intern","DutchDisclaimer":"<p>Deze publicatie is enkel beschikbaar voor persoonlijk gebruik binnen de Innovocean site <br />en mag op geen enkele manier verder worden verspreid.</p>","FileFormat":".pdf","FileDescr":"pdf","InsPub":1,"InsID":36,"FileFormID":6,"LendableFlag":1,"PublicFlag":1,"orderLib":"A","Notes":null,"AccConID":null,"AccessConstraint":null,"LicURL":null}],"litstyles":null,"thespers":null,"arch2discl":null,"SERpubls":[{"PublName":"Springer","City":"Heidelberg; Berlin"}],"MONpubls":null,"pictures":[],"thestermsPath":null,"thestermsASFA":null,"taxtermsASFA":[{"TaxTerm":"Bacteria"}],"geotermsASFA":null,"collections":[{"Collection":"VLIZ Acknowledged Publications","ShortName":"VLIZ ackn"}],"conf":null,"proj":null,"Physdatasets":null,"spcols":{"991":{"SpName":"EMBRC acknowledged","SpColID":991,"ParSpColID":null,"TopParID":null,"ShortName":"EMBRC ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"EMBRC ackn"},"1019":{"SpName":"EMBRC Acknowledged A1","SpColID":1019,"ParSpColID":991,"TopParID":991,"ShortName":"EMBRC Ackn A1","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":991,"SpColPath":"EMBRC ackn/EMBRC Ackn A1"},"39":{"SpName":"VLIZ Acknowledged Publications","SpColID":39,"ParSpColID":null,"TopParID":null,"ShortName":"VLIZ ackn","URLLocation":null,"LibID":36,"OpenRepoFlag":null,"SpTypID":null,"TopParIDNotWebsite":null,"SpColPath":"VLIZ ackn"}},"doi":null,"publs":null,"serparttypes":null,"monauthors":null,"MParts":null,"SParts":null,"hLibs":null,"langs":[{"BEntID":332757,"AbstractFlag":0,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"},{"BEntID":332757,"AbstractFlag":1,"LangID":15,"LangCode":"en","Lang":"English","DutchTerm":"Engels","LangCodeExtended":"eng"}],"urls":[{"URL":"https://dx.doi.org/10.1007/s00253-019-10082-z","externalID":"10.1007/s00253-019-10082-z","URLTypeCode":"DOI","URLID":92247,"URLTypID":13,"URLType":"DOI","URLPrefix":"http://dx.doi.org/"}],"thesterms":null,"taxterms":[{"TaxTerm":"Bacteria","AphiaID":6,"TaxtID":11315}],"geoterms":null,"othterms":null,"asfacodes":null,"asfa2codes":null,"thestermsFRIS":null,"taxtermsFRIS":[{"TaxTerm":"Bacteria","DutchTerm":null,"AphiaID":6,"TaxtID":11315}],"geotermsFRIS":null,"othtermsFRIS":null,"resmessage":"","complete":1,"sessions":{"newSesName":"Bouchti, Zohra, Z.","newSesDate":{"date":"2021-04-08 07:17:20.953000","timezone_type":3,"timezone":"Europe/Brussels"},"updSesName":"Lust, Heike, H.","updSesDate":{"date":"2022-12-07 09:09:02.657000","timezone_type":3,"timezone":"Europe/Brussels"}}}