{"refrec":{"BRefID":405835,"RR":"<b>Boyen, J.; Rodríguez, M.T.; Vlaeminck, B.; Fink, P.; Hablützel, P.I.; De Troch, M.</b> (2024). Temperature, pH, and diet interactively affect biosynthesis of polyunsaturated fatty acids in a benthic harpacticoid copepod. <i>Limnol. Oceanogr. 70(2)</i>: 334-348. <a href=\"https://dx.doi.org/10.1002/lno.12763\" target=\"_blank\">https://dx.doi.org/10.1002/lno.12763</a>","BEntID":403630,"PublicFlag":1,"CheckedFlag":1,"wosflag":1,"vabbflag":0,"RefStringPartII":". <i>Limnol. Oceanogr. 70(2)</i>: 334-348. <a href=\"https://dx.doi.org/10.1002/lno.12763\" target=\"_blank\">https://dx.doi.org/10.1002/lno.12763</a>","DocTypID":8,"DocType":"Journal article","MarineFlag":1,"FreshFlag":0,"BrackishFlag":0,"TerrestrialFlag":0,"Authorstring":"Boyen, J.; Rodríguez, M.T.; Vlaeminck, B.; Fink, P.; Hablützel, P.I.; De Troch, M.","OrigTitleTranslFlag":0,"Authorstringtrunc":"Boyen, J. <i>et al.</i>","Englishabstract":"Greenhouse gas emissions lead to ocean warming and acidification, negatively impacting marine organisms and their functioning, including long-chain polyunsaturated fatty acid (LC-PUFA) production by marine microalgae. Copepods, primary consumers of microalgae, possess a unique capacity for endogenous LC-PUFA biosynthesis, possibly enabling them to cope with reduced dietary LC-PUFA availabilities. However, this capacity may be itself impacted by changing oceanographic conditions. In this study, we conducted a laboratory experiment to evaluate the combined effects of warming (+3°C), acidification (−0.4 pH), and dietary LC-PUFA deficiency on the fatty acid composition and LC-PUFA biosynthesis (measured by quantitative RT-PCR) of the benthic harpacticoid copepod <i>Platychelipus littoralis</i> (Brady, 1880). We hypothesized increased LC-PUFA biosynthesis under all drivers compensating for LC-PUFA reductions. Lipid profiles of copepods exposed to multiple stressors contained shorter-chained and more saturated fatty acids. While copepods maintained base-line relative concentrations of the physiologically important LC-PUFA docosahexaenoic acid (DHA) on an LC-PUFA deficient diet at ambient temperatures, DHA concentrations decreased significantly with higher temperatures. Expression of the DHA biosynthesis genes Δ4 front-end desaturase and elovl1a increased under dietary LC-PUFA deficiency but did not exceed base-line levels when simultaneously exposed to acidification. Expression of Δ4 front-end desaturase and multiple elongases correlated positively with C<sub>18</sub> precursor concentrations and negatively with those of LC-PUFAs such as DHA, indicating their role as LC-PUFA biosynthesis enzymes. Overall, our findings suggest that ocean warming and acidification may impede benthic copepods' LC-PUFA biosynthesis capacity under reduced dietary inputs, limiting their contribution toward global LC-PUFA availability for higher trophic levels.","AbstractOtherLang":null,"BibLvlCode":"AS","StandardTitle":"Temperature, pH, and diet interactively affect biosynthesis of polyunsaturated fatty acids in a benthic harpacticoid copepod","OrigTitleLangCode":"en","OrigTitleLangCodeExtended":"eng","OrigTitleLangID":15,"DateLastModified":{"date":"2026-04-17 01:33:00.246134","timezone_type":1,"timezone":"+02:00"},"UserAccessRight":null,"UserAccID":null,"AuthorKeywords":"OCEAN ACIDIFICATION;CLIMATE-CHANGE;CO2;DESATURASES;SEAWATER;IMPACTS;GAS","OtherDescriptors":null,"Notes":null,"AnaPub":2024,"MonPub":null,"DateUpdate":"2025-09-26","DateCreate":"2025-02-25","SecASFANote":null,"ConfID":null,"PeerRev":1,"VlizCoreFlag":1,"WoScode":"WOS:001380603800001","VABBcode":null,"OpenAcc":0,"DOI":"10.1002/lno.12763"},"refs":null,"anarec":{"AnaID":405835,"PubliDate":2024,"Pagination":"334-348","XtraPublOfAnaID":null,"ISBN":null,"Volume":"70","Issue":"2","BRefMon":null,"BRefMonRR":null,"BRefXtra":null,"BRefXtraRR":null,"SerBRefID":43511,"SerRR":"Limnology and Oceanography. 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