Skip to main content

IMIS

A new integrated search interface will become available in the next phase of marineinfo.org.
For the time being, please use IMIS to search available data

 

[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [301309]
Quorum sensing negatively regulates chitinase in Vibrio harveyi
Defoirdt, T.; Ruwandeepika, H.; Karunasagar, I.; Boon, N.; Bossier, P. (2010). Quorum sensing negatively regulates chitinase in Vibrio harveyi. Environmental Microbiology Reports 2(1): 44-49. https://dx.doi.org/10.1111/j.1758-2229.2009.00043.x
In: Environmental Microbiology Reports. Wiley-Blackwell. ISSN 1758-2229, more
Peer reviewed article  

Available in  Authors 

Authors  Top 
  • Defoirdt, T., more
  • Ruwandeepika, H., more
  • Karunasagar, I.

Abstract
    Quorum sensing, bacterial cell‐to‐cell communication, regulates the virulence of Vibrio harveyi towards different hosts. Chitinase can be considered as a virulence factor because it helps pathogenic bacteria to attach to the host and to penetrate its tissues (e.g. in case of shrimp). Here, we show that quorum sensing negatively regulates chitinase in V. harveyi. Chitinolytic activity towards natural chitin from crab shells, the synthetic chitin derivative chitin azure, and fluorogenic chitin oligomers was significantly higher in a mutant in which the quorum‐sensing system is completely inactivated when compared with a mutant in which the system is maximally active. Furthermore, the addition of signal molecule containing cell‐free culture fluids decreased chitinase activity in a Harveyi Autoinducer 1 and Autoinducer 2‐deficient double mutant. Finally, chitinase A mRNA levels were fivefold lower in the mutant in which the quorum‐sensing system is maximally active when compared with the mutant in which the system is completely inactivated. [Correction added on 25 September 2009, after first online publication: the preceding sentence was corrected from ‘Finally, chitinase A mRNA levels were fivefold lower in the mutant in which the quorum‐sensing system is completely inactivated when compared with the mutant in which the system is maximally active.’] We argue that this regulation might help the vibrios to switch between host‐associated and free‐living life styles.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors