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Antarctic cryosphere fungal diversity (2015)
Citation
de Menezes G, Camara P, Pinto O, Convey P, Calvarho-Silva M, Simões J, Rosa C, Rosa L (2021): Antarctic cryosphere fungal diversity (2015). v1.8. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=cryosphere_fungi_antarctica_2015&v=1.8 https://doi.org/10.15468/wwbr57

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Archived data
Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution 4.0 International License.

Notes: The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.

Description
Amplicon sequencing dataset (Illumina MiSeq) targeting Fungi (ITS) in ice samples (n=8) from the Antarctic Peninsula. more

Samples were collected using sterile suits and gloves. Each sample was broken into smaller pieces, and surface decontamination carried out using 5% sodium hypochlorite (10 s), sterilized distilled water (10 s), and exposure to ultraviolet radiation (10 min).
Glacial ice fragments of approximately 20 kg mass, were collected adjacent to the ice fronts of seven marine terminating glaciers in the South Shetland Islands and the north-west Antarctica Peninsula during the austral summer season in December 2015 and December 2016.
The samples were melted and a total of 12–15 L of the resulting water Filtered through 47 mm diameter (Millipore) membranes (three membranes per sampling site, each using 4–5 L) until each membrane became saturated. Membranes were then stored at -20°C until DNA extraction.
The three membranes resulting from filtering the melted ice from each sampling site were processed together in order to increase DNA yield. Total DNA was extracted using 0.5 mL extraction buffer [sodium dodecyl sulfate (SDS) 10%], left at 55°C for 18 h, followed by 165 μL NaCl (5 M) and 165 μL cetyltrimethylammonium bromide (CTAB, 10%), then 600 μL chloroform was added and the mixture centrifuged (Eppendorf/Germany) at 13,000 rpm for 10 min. The supernatant was cleaned using the QIAGEN Dneasy PowerClean cleanup Kit. The ITS2 region was used as a DNA barcode, using the universal primers ITS3 and ITS4 and were sequenced at Macrogen Inc. (South Korea) on an Illumina MiSeq sequencer, using the MiSeq Reagent Kit v3 (600-cycle) following the manufacturer’s protocol.

Scope
Themes:
Biology > Ecology - biodiversity
Keywords:
Fresh water, Terrestrial, Glaciers, Metadata, Antarctica, Antarctic Peninsula, Fungi

Geographical coverage
Antarctica, Antarctic Peninsula Stations [Marine Regions]
maritime Antarctica

Temporal coverage
1 December 2015 - 31 December 2016

Taxonomic coverage
Fungi [WoRMS]

Parameter
DNA

Contributors
Federal University of Minas Gerais (UFMG), moredata creator
University of Brasilia (UnB), moredata creator
Natural Environment Research Council; British Antarctic Survey (BAS), moredata creator
Universidade Federal do Rio Grande do Sul (UFRGS), moredata creator

Related datasets
Published in:
AntOBIS: Antarctic Ocean Biodiversity Information System, more
(Partly) included in:
RAS: Register of Antarctic Species, more

Dataset status: Completed
Data type: Metadata
Data origin: Research: field experiment
Metadatarecord created: 2021-07-05
Information last updated: 2021-07-05
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy