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RGB-statistics derived from Nile red-stained reference plastics for the construction of the PDM (Plastics Detection Model) Citable as data publication Meyers, N.; Catarino, A.I.; Declercq, A. M.; Brenan, A.; Devriese, L.; Vandegehuchte, M.; De Witte, B.; Janssen, C.; Everaert, G.; Flanders Marine Institute (VLIZ); Flanders Research Institute for Agriculture, Fisheries and Food (ILVO); Ghent University Laboratory for Environmental Toxicology (GhEnToxLab): Belgium; (2021): RGB-statistics derived from Nile red-stained reference plastics for the construction of the PDM (Plastics Detection Model). Marine Data Archive. https://doi.org/10.14284/512 Contact: Meyers, Nelle Availability: This dataset is licensed under a Creative Commons Attribution 4.0 International License. Description Dataset containing RGB-statistics extracted from photographed fluorescent reference particles stained with Nile red. The most abundantly produced plastic polymers worldwide as well as natural materials with high prevalence in the marine environment were considered for this dataset. The spectral data was used to construct a supervised machine learning model that allows to accurately distinguish plastic from natural particles in a cost- and time-efficient way. more The dataset was built to train and validate the ‘Plastic Detection Model’ (PDM) in R and contains Red, Green and Blue (RGB) statistics extracted from Nile red-stained reference particles (50-1200 μm) photographed under three different microscope filters (UV: Filter System A S, BP 340-380 nm; blue: Filter System I3 S, BP 450-490 nm; and green: Filter system N2.1 S, BP 515-560 nm) (LEICA DM 1000). Image analysis to extract all RGB-values was performed using a macro in ImageJ. The supervised machine learning model (CART algorithm) trained by and validated with this dataset predicts with high accuracy the plastic or non-plastic, natural origin of particles, in a cost- and time-efficient way. RGB statistics of the most abundantly produced plastic polymers worldwide as well as natural materials with high prevalence in the marine environment were compiled into the dataset. The statistics itself were calculated per reference particle as the 10th, 50th and 90th percentile as well as the mean of each of the three different color components extracted from all pixels laying along the maximum Feret diameter of that photographed particle. The dataset contains RGB-statistics calculated through image analysis of 60 plastic and 60 non-plastic particles, where 96 particles (4/5) were randomly selected and used to serve as training data (worksheet tab ‘training data’), while the remaining 24 particles (1/5) were kept as independent validation data (worksheet tab ‘validation data’). Scope Themes: Environmental quality/pollution Keywords: Marine/Coastal, Fresh water, Brackish water, Detection method, Fluorescence microscopy, Machine learning, Microplastics, RGB colour data, World Geographical coverage World [Marine Regions] Parameter RGB (Red, Green, Blue) colour component means and percentiles Methodology RGB (Red, Green, Blue) colour component means and percentiles: Fluorescence microscopy combined with image analysis. Contributors Vlaams Instituut voor de Zee (VLIZ), more, data creator, data creator Instituut voor Landbouw-, Visserij- en Voedingsonderzoek (ILVO), more, data creator Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Milieutoxicologie (GhEnToxLab), more, data creator Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Dierwetenschappen en Aquatische Ecologie; Laboratorium voor Aquacultuur en Artemia Reference Center (ARC), more, data creator Related datasets Project PhD Developing and optimising cost- and time-effective methods for the detection and identification of microplastics in the marine environment, more Funding OFI ANDROMEDA: Analysis techniques for quantifying nano-and microplastic particles and their degradation in the marine environment, more Funding BRAIN-be(Belgian Research Action through Interdisciplinary Networks) Dataset status: Completed Data type: Data Data origin: Research: lab experiment Metadatarecord created: 2021-08-23 Information last updated: 2021-09-28 |