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Viral infection arrests microalgae-host calcification and nutrient consumption, and triggers shifts in organic stoichiometry
Citable as data publication
Frada, M.; The Hebrew University of Jerusalem (HUJI): Israel; (2023): Viral infection arrests microalgae-host calcification and nutrient consumption, and triggers shifts in organic stoichiometry. Marine Data Archive. https://doi.org/10.14284/621
Contact: Frada, Miguel

Availability: Creative Commons License This dataset is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Description

Blooms of the dominant coccolithophore Emiliania huxleyi are routinely infected by a specific lytic virus (EhV), which rapidly kills host cells triggering bloom termination and organic and inorganic carbon export. However, the impact of EhV on the dynamic of resource acquisition and cellular stoichiometry remains unknown, limiting the current understanding of the ecological and biogeochemical significance of E. huxleyi blooms. To tackle this knowledge gap, we used algal and EhV cultures to determine over the course of infections the dynamics of alkalinity, modulated by calcification, nitrate and phosphate consumption and organic matter stoichiometry. - Raw data used for this paper https://doi.org/10.1101/2023.07.11.548577

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We found that within 24hr alkalinity concentration stabilized and nutrient uptake declined to background levels. In parallel, the stoichiometric ratio of carbon to nitrogen increased and the nitrogen to phosphorus ratio declined. These variations likely resulted from lipid accumulation required for viral replication and the differential retention of phosphorus-rich macromolecular pools in decaying cells, respectively. Finally, after host population decay, a progressive enrichment in phosphorus relative to nitrogen and carbon was detected in the remaining cell lysates. We estimate that this post-infection stoichiometric shift was driven by the accumulation of heterotrophic bacteria involved in the degradation of organic material. Viral-mediated cell remodeling and consequent shifts in biomass stoichiometry likely impacts the patterns of nutrient cycling and biological carbon pump efficiency during large-scale blooms in the oceans.


Scope
Themes:
Biology > Plankton
Keywords:
Alkalinity, Bacteria, Nutrient stoichiometry, Nutrients (mineral), Viruses, Emiliania huxleyi (Lohmann) W.W.Hay & H.Mohler, 1967

Temporal coverage
1 January 2003 - 31 December 2003
Not relevant

Taxonomic coverage
Emiliania huxleyi (Lohmann) W.W.Hay & H.Mohler, 1967 [WoRMS]

Parameters
Alkalinity Methodology
autofluorescence of Emiliana huxleyi Methodology
Count of bacteria Methodology
Counts of EhV virus Methodology
Counts of Emiliana huxleyi Methodology
Nitrate (NO3-) Methodology
Particulate organic carbon (POC) Methodology
Particulate organic nitrogen (PON) Methodology
particulate organic phosphorus Methodology
Phosphate (PO43-) Methodology
Side scatter of Emiliana huxleyi Methodology
Alkalinity: acid-base titration
acid-base titration
autofluorescence of Emiliana huxleyi: Flow cytometry

Count of bacteria: Flow cytometry

Counts of EhV virus: Flow cytometry

Counts of Emiliana huxleyi: Flow cytometry

Nitrate (NO3-): second derivative spectroscopy
second derivate spectroscopy

Nitrate (NO3-): second derivative spectroscopy
second derivate spectroscopy
Particulate organic carbon (POC): Continuous flow elemental analysis isotope ratio mass spectrometry (CF-EA-IRMS)
Particulate organic nitrogen (PON): Continuous flow elemental analysis isotope ratio mass spectrometry (CF-EA-IRMS)
particulate organic phosphorus: colorimetric spectroscopy (Mo-blue method)
colorimetric spectroscopy using the molybdenum blue method
Phosphate (PO43-): colorimetric spectroscopy (Mo-blue method)
colorimetric spectroscopy using the molybdenum blue method

Phosphate (PO43-): colorimetric spectroscopy (Mo-blue method)
colorimetric spectroscopy using the molybdenum blue method
Side scatter of Emiliana huxleyi: Flow cytometry


Contributors
The Hebrew University of Jerusalem (HUJI), moredata creator

Dataset status: Completed
Data type: Data
Data origin: Research: lab experiment
Metadatarecord created: 2023-08-22
Information last updated: 2023-09-08
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy