Non-target effects of the insecticide methoprene on molting in the estuarine crustacean Neomysis integer (Crustacea: Mysidacea)
Ghekiere, A.; Verslycke, T.; Fockedey, N.; Janssen, C.R. (2006). Non-target effects of the insecticide methoprene on molting in the estuarine crustacean Neomysis integer (Crustacea: Mysidacea). J. Exp. Mar. Biol. Ecol. 332(2): 226-234. dx.doi.org/10.1016/j.jembe.2005.11.021 In: Journal of Experimental Marine Biology and Ecology. Elsevier: New York. ISSN 0022-0981; e-ISSN 1879-1697, more |  |
| Keywords | Biological phenomena > Metamorphosis > Moulting
Developmental stages > Juveniles
Growth regulators > Insecticides > Insect growth regulators > Juvenile hormone analogues > Methoprene
Secretory organs > Glands > Endocrine glands
Secretory products > Hormones
Neomysis integer (Leach, 1814) [WoRMS]; Neomysis integer (Leach, 1814) [WoRMS]
Marine/Coastal; Brackish water | | Author keywords | endocrine disruption; juvenile hormone analog; molting; methoprene; Neomysis integer |
| Abstract | Ecdysteroids, the molting hormones in crustaceans and other arthropods, play a crucial role in the control of growth, reproduction and embryogenesis of these organisms. Insecticides are often designed to target specific endocrine-regulated functions such as molting and larval development such as methoprene, a juvenile hormone analogue.The aim of this study was to examine the effects of methoprene on molting in a non-target species, the estuarine mysid Neomysis integer (Crustacea: Mysidacea). Mysids have been proposed as standard test organisms for evaluating the endocrine disruptive effect of chemicals. Juveniles (< 24 h) were exposed for 3 weeks to the nominal concentrations 0.01, 1 and 100 µg methoprene/l. Daily, present molts were checked and stored in 4% formaldehyde for subsequent growth measurements. Methoprene significantly delayed molting at 100 µg/l by decreasing the growth rate and increasing the intermolt period. This resulted in a decreased wet weight of the organism. The anti-ecdysteroidal properties of methoprene on mysid molting were also evaluated by determining the ability of exogenously administered 20-hydroxyecdysone, the active ecdysteroid in crustaceans, to protect against the observed methoprene effects. Co-exposure to 20-hydroxyecdysone did not mitigate methoprene effects on mysid molting. This study demonstrates the need for incorporating invertebrate-specific hormone-regulated endpoints in regulatory screening and testing programs for the detection of endocrine disruption caused by man-made chemicals. |
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