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Analysis of Flow Cytometry Data
Sharrow, S.O. (2001). Analysis of Flow Cytometry Data, in: Coligan, J.E. et al. (1991-2017). Current Protocols in Immunology. pp. UNIT 5.2: 5.2.1-5.2.10. https://dx.doi.org/10.1002/0471142735.im0502s00
In: Coligan, J.E. et al. (Ed.) (1991-2017). Current Protocols in Immunology. Wiley Online Library: London. ISBN 978-0-47-114273-7. https://dx.doi.org/10.1002/0471142735, more
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Keywords
    Flow cytometry
    Protocols
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  • Sharrow, S.O.
Abstract
    This unit provides several approaches to flow cytometry data analysis. Frequency determinations based on analysis of single-parameter fluorescence histograms and dual-parameter contour plots are presented. Next, steps are described for calculating values for signal-to-noise ratios when logarithmic amplification is used for data collection. This calculation can be used to compare the amounts of antigenic determinants per cell between different cells stained with the same reagent. Finally, a procedure is described for constructing a calibration curve for logarithmic amplifiers. This calibration curve is required for calculation of signal-to-noise values and can also be used to determine if the amplifiers are working properly.
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