A microfabricated, flow-driven grinding mill for mechanical cell lysing
Smith, R.L.; England, A.; Millis, J.; Hirn, C.; Collins, S.D.; Connell, L.B. (2023). A microfabricated, flow-driven grinding mill for mechanical cell lysing. Anal. Chem. 95(48): 17494-17501. https://dx.doi.org/10.1021/acs.analchem.3c02344
In: Analytical chemistry. American Chemical Society: Washington. ISSN 0003-2700; e-ISSN 1520-6882, more
|  |
| Keyword |
Alexandrium Halim, 1960 [WoRMS]
|
| Authors | | Top |
- Smith, R.L.
- England, A.
- Millis, J.
|
- Hirn, C.
- Collins, S.D.
- Connell, L.B.
|
|
| Abstract |
This paper presents the design, microfabrication, and demonstration of a novel microfluidic grinding mill for the lysis of the dinoflagellate, Alexandrium, a neurotoxin-producing genus of algae that is responsible for red tide and paralytic shellfish poisoning. The mill consists of a high-speed, hydrodynamically driven microrotor coupled to a micro grinding mill that lyses robust algal cells by mechanical abrasion with single-pass efficiencies as high as 97%. These efficiencies are comparable to, or better than, current mechanical and chemical lysing methods without adding complications associated with harsh chemical additives that can interfere with subsequent downstream bioanalysis. Release of cytoplasm from lysed algae was confirmed using polymerase chain reaction (PCR) amplification of Alexandrium DNA using dinoflagellate primers. |
|
