Microarrays/DNA chips for the detection of waterborne pathogens
Vale, F. (2016). Microarrays/DNA chips for the detection of waterborne pathogens, in: Bourlat, S.J. (Ed.) Marine genomics: methods and protocols. Methods in Molecular Biology, 1452: pp. 143-153. https://dx.doi.org/10.1007/978-1-4939-3774-5_9
In: Bourlat, S.J. (Ed.) (2016). Marine genomics: methods and protocols. Methods in Molecular Biology, 1452. Humana Press/Springer Science+Business Media, Inc: New York. ISBN 978-1-4939-3772-1. 253 pp. https://dx.doi.org/10.1007/978-1-4939-3774-5, more
In: Methods in Molecular Biology. Humana Press. ISSN 1064-3745, more
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| Author keywords |
DNA chip; Microarray; Waterborne pathogen; Waterborne detection; Molecular method; Water quality; DNA probe; Bacterial detection; Indicator bacteria; Microbial detection |
| Abstract |
DNA microarrays are useful for the simultaneous detection of microorganisms in water samples. Specific probes targeting waterborne pathogens are selected with bioinformatics tools, synthesized and spotted onto a DNA array. Here, the construction of a DNA chip for waterborne pathogen detection is described, including the processes of probe in silico selection, synthesis, validation, and data analysis. |
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