Genetic analisys of cytochrome oxidase sub unit 1 gene fragment from Cirrhilabrus cf. ryukyuensis (Labridae) from Cenderawasih Bay and Raja Ampat
Dailami, M.; Santi, D.; Murtihapsari; Abubakar, H.; Toha, A.H.A. (2018). Genetic analisys of cytochrome oxidase sub unit 1 gene fragment from Cirrhilabrus cf. ryukyuensis (Labridae) from Cenderawasih Bay and Raja Ampat. J. Iktiol. Indones. 18(3): 209-222. https://dx.doi.org/10.32491/jii.v18i3.347
In: Jurnal Iktiologi Indonesia. Masyarakat Iktiologi Indonesia: Bogor. ISSN 1693-0339; e-ISSN 2579-8634, more
|  |
| Keyword |
Cirrhilabrus ryukyuensis Ishikawa, 1904 [WoRMS]
|
| Author keywords |
Cirrhilabrus cf ryukyuensis; Gen COI; Filogenetik; wrase |
| Authors | | Top |
- Dailami, M.
- Santi, D.
- Murtihapsari
|
- Abubakar, H.
- Toha, A.H.A.
|
|
| Abstract |
Cirrhilabrus cf. ryukyuensis from Cenderawasih Bay and Raja Ampat have different colormorphs with Cirrhilabrus ryukyuensis. This study was conducted to determine the phylogenetic relationship, evolution and genetic diversity of Cirrhilabrus cf. ryukyuensis based on Cytochrome oxidase sub unit 1 (COI) gene. Mitochondrial DNA extracted by using chelex 10%. FISH-BCH and BCL primers were used to amplify COI gene fragment and sequencing. Data analysis conducted by using software Sequencher 4.1 and MEGA 5. COI genes from six samples of C. cf rykyuensis from Cenderawasih Bay and Raja Ampat have total 613 base pairs. The average compositions of nucleotides are T 32.8%, C 26.3%, A 23.2% and G 17.7%. There is six positions of polymorphism that caused by transition. Conversion of nucleotides to amino acid sequence resulted in 204 amino acid with the same sequence. The phylogenetic tree from six sample showed that all in one clade, but different clade from Cirrhilabrus cyanopleura from GenBank |
|
