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A metabolomics guided exploration of marine natural product chemical space
Floros, D.J.; Jensen, P.R.; Dorrestein, P.C.; Koyama, N. (2016). A metabolomics guided exploration of marine natural product chemical space. Metabolomics 12(9): 145. https://dx.doi.org/10.1007/s11306-016-1087-5
In: Metabolomics. Springer: New York. ISSN 1573-3882; e-ISSN 1573-3890, more
Peer reviewed article  
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Keyword
    Marine/Coastal
Author keywords
    Metabolomics; Marine natural products; Mass spectrometry; Molecular networking
Authors  Top 
  • Floros, D.J.
  • Jensen, P.R.
  • Dorrestein, P.C.
  • Koyama, N.
Abstract

    Introduction

    Natural products from culture collections have enormous impact in advancing discovery programs for metabolites of biotechnological importance. These discovery efforts rely on the metabolomic characterization of strain collections.

    Objective

    Many emerging approaches compare metabolomic profiles of such collections, but few enable the analysis and prioritization of thousands of samples from diverse organisms while delivering chemistry specific read outs.

    Method

    In this work we utilize untargeted LC–MS/MS based metabolomics together with molecular networking to inventory the chemistries associated with 1000 marine microorganisms.

    Result

    This approach annotated 76 molecular families (a spectral match rate of 28 %), including clinically and biotechnologically important molecules such as valinomycin, actinomycin D, and desferrioxamine E. Targeting a molecular family produced primarily by one microorganism led to the isolation and structure elucidation of two new molecules designated maridric acids A and B.

    Conclusion

    Molecular networking guided exploration of large culture collections allows for rapid dereplication of know molecules and can highlight producers of uniques metabolites. These methods, together with large culture collections and growing databases, allow for data driven strain prioritization with a focus on novel chemistries.

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