Skip to main content

IMIS

A new integrated search interface will become available in the next phase of marineinfo.org.
For the time being, please use IMIS to search available data

 

[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [130019]
Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
Pujolar, J.M.; Maes, G.E.; Van Houdt, J.K.J.; Zane, L. (2009). Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla). Mol. Ecol. Resour. 9(1): 233-235. dx.doi.org/10.1111/j.1755-0998.2008.02419.x
In: Molecular Ecology Resources. Blackwell Publishing: Oxford. ISSN 1755-098X; e-ISSN 1755-0998, more
Peer reviewed article  

Available in  Authors 

Keywords
    Microsatellites
    Sequences
    Anguilla anguilla (Linnaeus, 1758) [WoRMS]
    Marine/Coastal
Author keywords
    Anguilla; EST; European eel; microsatellite

Authors  Top 
  • Pujolar, J.M.
  • Maes, G.E., more
  • Van Houdt, J.K.J., more
  • Zane, L., more

Abstract
    A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata).

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors