IMIS

A new integrated search interface will become available in the next phase of marineinfo.org.
For the time being, please use IMIS to search available data

 

[ report an error in this record ]basket (1): add | show Print this page
one publication added to basket [261818]
Identification of one tachykinin- and two kinin-related peptides in the brain of the white shrimp, Penaeus vannamei
Nieto, J.; Veelaert, D.; Derua, R.; Waelkens, E.; Cerstiaens, A.; Coast, G.; Devreese, B.; Van Beeumen, J.; Calderon, J.; De Loof, A.; Schoofs, L. (1998). Identification of one tachykinin- and two kinin-related peptides in the brain of the white shrimp, Penaeus vannamei. Biochem. Biophys. Res. Commun. 248(2): 406-411. https://dx.doi.org/10.1006/bbrc.1998.8964
In: Biochemical and Biophysical Research Communications. ACADEMIC PRESS INC ELSEVIER SCIENCE: San Diego etc.. ISSN 0006-291X; e-ISSN 1090-2104, more
Peer reviewed article  
Available in  Authors 
Keywords
    Penaeus vannamei Boone, 1931 [WoRMS]
    Marine/Coastal
Authors  Top 
  • Nieto, J.
  • Veelaert, D.
  • Derua, R., more
  • Waelkens, E., more
  • Cerstiaens, A.
  • Coast, G.
  • Devreese, B.
  • Van Beeumen, J., more
  • Calderon, J.
  • De Loof, A.
  • Schoofs, L.
Abstract
    This paper reports the purification of three myotropic neuropeptides from the white shrimp Penaeus vannamei.The central nervous systems of 3500 shrimps were extracted in an acidified solvent, after which four to five HPLC column systems were used to obtain pure peptides. A cockroach hindgut muscle contraction bioassay was used to monitor all collected fractions. The pure peptides were submitted to Edman degradation based automated microsequencing. Mass spectrometry and chemical synthesis confirmed the sequences. Ala-Pro-Ser-Gly-Phe-Leu-Gly-Met-Arg-NH2(Pev-tachykinin, 934.1 Da) belongs to the tachykinin family with identified members in all vertebrate classes and some invertebrate classes: arthropods, annelids and molluscs. A very specific Pev-tachykinin antiserum was developed, which labels 4 neurosecretory cells in the brain. Ala-Ser-Phe-Ser-Pro-Trp-Gly-NH2(Pev-kinin 1, 749.8 Da) and Asp-Phe-Ser-Ala-Trp-Ala-NH2(Pev-kinin 2, 694.7 Da) are the first crustacean kinins. Pev-kinin 2 is the first kinin with a Trp-Ala-NH2instead of a kinin-typical Trp-Gly-NH2carboxyterminus.
All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors