Skip to main content

IMIS

A new integrated search interface will become available in the next phase of marineinfo.org.
For the time being, please use IMIS to search available data

 

[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [289256]
Monoclonal antibodies directed to fucoidan preparations from brown algae
Torode, T.A.; Marcus, S.E.; Jam, M.; Tonon, T.; Blackburn, R.S.; Hervé, C.; Knox, J.P. (2015). Monoclonal antibodies directed to fucoidan preparations from brown algae. PLoS One 10(2): e0118366. https://dx.doi.org/10.1371/journal.pone.0118366
In: PLoS One. Public Library of Science: San Francisco. ISSN 1932-6203; e-ISSN 1932-6203, more
Peer reviewed article  

Available in  Authors 

Keywords
    Marine Sciences
    Maritime Industries > Blue Biotech
    Scientific Community
    Scientific Publication
    Marine/Coastal

Project Top | Authors 
  • Association of European marine biological laboratories, more

Authors  Top 
  • Torode, T.A.
  • Marcus, S.E.
  • Jam, M.
  • Tonon, T.
  • Blackburn, R.S.
  • Hervé, C.
  • Knox, J.P.

Abstract
    Cell walls of the brown algae contain a diverse range of polysaccharides with useful bioactivities. The precise structures of the sulfated fucan/fucoidan group of polysaccharides and their roles in generating cell wall architectures and cell properties are not known in detail. Four rat monoclonal antibodies, BAM1 to BAM4, directed to sulfated fucan preparations, have been generated and used to dissect the heterogeneity of brown algal cell wall polysaccharides. BAM1 and BAM4, respectively, bind to a non-sulfated epitope and a sulfated epitope present in the sulfated fucan preparations. BAM2 and BAM3 identified additional distinct epitopes present in the fucoidan preparations. All four epitopes, not yet fully characterised, occur widely within the major brown algal taxonomic groups and show divergent distribution patterns in tissues. The analysis of cell wall extractions and fluorescence imaging reveal differences in the occurrence of the BAM1 to BAM4 epitopes in various tissues of Fucus vesiculosus. In Ectocarpus subulatus, a species closely related to the brown algal model Ectocarpus siliculosus, the BAM4 sulfated epitope was modulated in relation to salinity levels. This new set of monoclonal antibodies will be useful for the dissection of the highly complex and yet poorly resolved sulfated polysaccharides in the brown algae in relation to their ecological and economic significance.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors