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Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish
Tiago, D.M.; Marques, C.L.; Roberto, V.P.; Cancela, M.L.; Laizé, V. (2014). Mir-20a regulates in vitro mineralization and BMP signaling pathway by targeting BMP-2 transcript in fish. Arch. Biochem. Biophys. 543: 23-30. https://dx.doi.org/10.1016/j.abb.2013.12.009
In: Archives of biochemistry and biophysics. ELSEVIER SCIENCE INC: San Diego, CA,. ISSN 0003-9861; e-ISSN 1096-0384, more
Peer reviewed article  
Available in  Authors 
Keywords
    Marine Sciences
    Marine Sciences > Marine Genomics
    Scientific Community
    Scientific Publication
    Marine/Coastal
Author keywords
    BMP-2; Mineralizing fish bone-derived cells miR-20a;Post-transcriptional regulation; MGP-BMP-2 interaction
Project Top | Authors 
  • Association of European marine biological laboratories, more
Authors  Top 
  • Tiago, D.M.
  • Marques, C.L.
  • Roberto, V.P.
  • Cancela, M.L.
  • Laizé, V.
Abstract
    MicroRNAs (miRNAs) are important regulators of vertebrate development but their role during skeletogenesis remains unknown. In this regard, we investigated the mineralogenic activity of miR-20a, a miRNA associated with osteogenesis, in fish bone-derived cells. Expression of miR-20a was up-regulated during differentiation and its overexpression inhibited mineralization, suggesting a role in fish tissue calcification. In this regard, a conserved miR-20a binding site was identified in bone morphogenetic protein 2 (BMP-2) 3'UTR and its functionality was evidenced through luciferase assays, and further confirmed by western-blot and qPCR. Type II BMP receptor (BMPR2) is also targeted by miR-20a in mammalian systems and evidence was collected for the presence of a binding site in fish sequences. We propose that miR-20a is a regulator of BMP pathway through specific action on BMP-2 and possibly BMPR2. Overexpression of miR-20a was also shown to up-regulate matrix Gla protein (MGP) transcript, a physiological inhibitor of calcification previously found to form a complex with BMP-2. We propose that MGP may play a role in the anti-mineralogenic effect promoted by miR-20a by decreasing availability of BMP-2. This study gives new insights into miRNA-mediated regulation of BMP-2, and sheds light into the potential role of miR-20a as a regulator of skeletogenesis.
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