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A standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of the European sea bass Dicentrarchus labrax L.: comparison of extruded and extruded-coated diets
Coutteau, P.; Van Stappen, G.; Sorgeloos, P. (1996). A standard experimental diet for the study of fatty acid requirements of weaning and first ongrowing stages of the European sea bass Dicentrarchus labrax L.: comparison of extruded and extruded-coated diets. Arch. Anim. Nutr. 49: 49-59. https://dx.doi.org/10.1080/17450399609381863
In: Archives of Animal Nutrition. Taylor & Francis: Abingdon. ISSN 1745-039X; e-ISSN 1477-2817, more
Peer reviewed article  

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Keywords
    Dicentrarchus labrax (Linnaeus, 1758) [WoRMS]
    Marine/Coastal

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Abstract
    The stability of the coated lipid fraction of a standard weaning diet was verified by comparing growth and fatty acid incorporation for European sea bass fed diets with an identical formulation but prepared either by extrusion/coating or extrusion only. For each type of experimental diet, a (n-3) HUFA level of 1 and 2.5% of the dry diet was evaluated. European sea bass Dicentrarchus labrax L. were weaned and consequently reared for 23 days on the experimental diets in a recirculation system. A commercial weaning diet served as a control. Final individual dry weight of sea bass larvae weaned and ongrown for a total period of 30 days on the various diets did not differ significantly. The (n-3) HUFA content of the fish tissue was a reflection of the dietary (n-3) HUFA content and was not influenced by the technique used to include the essential fatty acids in the diet. The (n-3) HUFA requirements of European sea bass during and immediately after weaning did not exceed 1% of the dry diet. The combined technique of cooking-extrusion of a basal diet followed by coating of a lipid emulsion proved to be a valid technique to prepare diets for studying quantitative fatty acid requirements of weaning and first ongrowing stages of marine fish. A standard diet with open formulation is proposed.

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